Mora-Ocación Mary S, Morillo-Coronado Ana Cruz, Manjarres-Hernández Elsa Helena
Escuela de Ingeniería agronómica, Universidad Pedagógica y Tecnológica de Colombia, Tunja 150003, Colombia.
Escuela de Ciencias Biológicas, Universidad Pedagógica y Tecnológica de Colombia, Tunja 150003, Colombia.
Int J Food Sci. 2022 Feb 28;2022:7287487. doi: 10.1155/2022/7287487. eCollection 2022.
Quinoa has a high nutraceutical potential because of the presence of secondary metabolites called saponins, which have industrial and medicinal uses and protect against attacks by pathogens. These compounds are found especially in the seed coat and give the grain a bitter taste; therefore, they must be eliminated before consumption. Despite the potential use in Colombia, there are few studies aimed at quantifying this metabolite. Therefore, the objective of this research was to evaluate two extraction methodologies (physical and chemical) and two methods for quantifying saponins in five quinoa genotypes grown in Colombia. The most efficient extraction method was the physical method. The saponin contents of the five genotypes were variable. The cluster analysis differentiated the genotypes into two groups: low saponin content (<4.49 mg/g seed) and high saponin content (>14.76 mg/g seeds). Blanca de Jericó had the lowest saponin content (<0.40%), and Amarilla de Maranganí had the highest content (>0.18%). Identifying more efficient methodologies for extracting and quantifying saponins will allow a better characterization of the germplasm and selection of genotypes with desirable characteristics for both consumption and industrial use.
藜麦具有很高的营养保健潜力，因为其含有一种名为皂苷的次生代谢产物，皂苷具有工业和药用价值，并能抵御病原体的侵袭。这些化合物尤其存在于种皮中，会使谷物带有苦味；因此，在食用前必须将其去除。尽管藜麦在哥伦比亚有潜在的用途，但旨在量化这种代谢产物的研究却很少。因此，本研究的目的是评估两种提取方法（物理法和化学法）以及两种用于量化在哥伦比亚种植的五种藜麦基因型中皂苷含量的方法。最有效的提取方法是物理法。这五种基因型的皂苷含量各不相同。聚类分析将这些基因型分为两组：低皂苷含量（<4.49毫克/克种子）和高皂苷含量（>14.76毫克/克种子）。耶利哥白藜麦的皂苷含量最低（<0.40%），马拉甘尼黄藜麦的含量最高（>0.18%）。确定更有效的皂苷提取和量化方法将有助于更好地鉴定种质，并选择具有适合食用和工业用途的理想特性的基因型。
