Na Lu, Gao Jun-Feng, Liu Guo-Hua, Fu Xue, Su Xin, Yue Dong-Mei, Gao Yuan, Zhang Yan, Wang Chun-Ren
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China.
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China; Department of Parasitology, Heilongjiang Institute of Veterinary Science, Qiqihar, Heilongjiang Province 161006, PR China.
Infect Genet Evol. 2016 Apr;39:45-50. doi: 10.1016/j.meegid.2016.01.010. Epub 2016 Jan 13.
Metorchis orientalis (Trematoda: Opisthorchiidae) is an important trematode infecting many animals and humans, causing metorchiasis. In the present study, the complete mitochondrial (mt) genome of M. orientalis was sequenced. The complete mt genome of M. orientalis is 13,834 bp circular DNA molecule and contains 12 protein-coding genes, 22 transfer RNA genes, and two ribosomal RNA genes. The gene content and arrangement of M. orientalis is the same as those of Opisthorchiidae trematodes (Opisthorchis viverrini, Opisthorchis felineus and Clonorchis sinensis), but distinct from Schistosoma spp. Phylogenetic analyses using concatenated amino acid sequences of 12 protein-coding genes with three different computational algorithms (Bayesian inference, maximum likelihood and maximum parsimony) revealed that M. orientalis and O. viverrini represent sister taxa. The mt genome provides a novel genetic marker for further studies of the identification, classification and molecular epidemiology of Opisthorchiidae trematodes, and should have implications for the diagnosis, prevention and control of metorchiasis in animals and humans.
东方次睾吸虫（吸虫纲：后睾科）是一种重要的吸虫，可感染多种动物和人类，引发次睾吸虫病。在本研究中，对东方次睾吸虫的完整线粒体（mt）基因组进行了测序。东方次睾吸虫的完整mt基因组是一个13,834 bp的环状DNA分子，包含12个蛋白质编码基因、22个转运RNA基因和两个核糖体RNA基因。东方次睾吸虫的基因内容和排列与后睾科吸虫（麝猫后睾吸虫、猫后睾吸虫和华支睾吸虫）相同，但与血吸虫属不同。使用三种不同计算算法（贝叶斯推断、最大似然法和最大简约法）对12个蛋白质编码基因的串联氨基酸序列进行系统发育分析，结果显示东方次睾吸虫和麝猫后睾吸虫为姐妹类群。该mt基因组为后睾科吸虫的鉴定、分类和分子流行病学的进一步研究提供了一种新的遗传标记，并且对动物和人类次睾吸虫病的诊断、预防和控制具有重要意义。
