Wang Ping, Zhao Xiaomei, Qu Tianming, Liang Lijiao, Ji Qinglong, Chen Ying
Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
Foods. 2022 Feb 28;11(5):719. doi: 10.3390/foods11050719.
This study was undertaken to investigate the prevalence, antimicrobial resistance, and virulence gene profiles of in different brands of infant formula in Beijing supermarkets. Eighty-eight isolates were recovered in sixty-eight infant formulas of five domestic brands and fourteen imported brands. The prevalence rate in domestic and imported samples were 70.6% and 52.9%, respectively. Lower mean prevalence level was found in domestic samples (1.17 MPN/g) compared with the imported samples (3.52 MPN/g). Twenty-four virulence gene profiles were found, and most strains carried at least one virulence gene. The prevalence of , , , , and in domestic and imported brand samples was similar. The occurrence of enterotoxin genes , and in domestic samples were 22.2%, 27.8%, and 22.2%, respectively, which was significantly higher than imported samples. Antimicrobial drugs-susceptibility analysis showed that all isolates were susceptible to gentamincin, amikacin, and ciprofloxacin; 38%, 7%, and 2.3% were resistant to rifampin, tetracycline, and chloramphenicol, respectively; and only one isolate was resistant to trimethoprim-sulfamethoxazole. Moreover, the cell numbers of in prepared infant formula increased rapidly at room temperature. Thus, monitoring guidelines are needed for accepted levels of in infant formula.
本研究旨在调查北京超市不同品牌婴儿配方奶粉中[具体细菌名称未给出]的流行情况、抗菌药物耐药性和毒力基因谱。从五个国产品牌和十四个进口品牌的六十八种婴儿配方奶粉中分离出八十八株[具体细菌名称未给出]。国产和进口样品中的检出率分别为70.6%和52.9%。与进口样品(3.52 MPN/g)相比,国产样品中[具体细菌名称未给出]的平均检出水平较低(1.17 MPN/g)。发现了二十四种毒力基因谱,大多数菌株携带至少一种毒力基因。国产和进口品牌样品中[具体细菌名称未给出]、[具体细菌名称未给出]、[具体细菌名称未给出]、[具体细菌名称未给出]、[具体细菌名称未给出]和[具体细菌名称未给出]的流行情况相似。国产样品中肠毒素基因[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]的检出率分别为22.2%、27.8%和22.2%,显著高于进口样品。抗菌药物敏感性分析表明,所有分离株对庆大霉素、阿米卡星和环丙沙星敏感;分别有38%、7%和2.3%的分离株对利福平、四环素和氯霉素耐药;仅一株分离株对甲氧苄啶-磺胺甲恶唑耐药。此外,在室温下,配制好的婴儿配方奶粉中[具体细菌名称未给出]的细胞数量迅速增加。因此,需要制定婴儿配方奶粉中[具体细菌名称未给出]可接受水平的监测指南。