Liang Weibing, Chen Ming, Li Lin, Yan Liqiang, Wang Xiuli, Wu Xiongzhi, Lei Chenghong
College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541006, China.
Polymers (Basel). 2022 Feb 28;14(5):959. doi: 10.3390/polym14050959.
In this work, the positively-charged polymer polyethyleneimine was used to functionalize carbon nanotubes and activated carbon to load antimycotic enzyme lyticase. Interestingly, polyethyleneimine played a dual role functionalizing carbon materials to synergistically enhance antimycotic activity of loaded lyticase as well as exhibiting its own apparent antimycotic activity, where the enhanced enzymatic activity of loaded lyticase on functionalized carbon nanotubes was more than 2.8 times as high as the activity of free enzyme in solution. The actual activity of loaded lyticase on functionalized carbon nanotubes was applied with , exhibiting much faster digesting lysis of the bacteria in comparison with free lyticase. The synergistic and potent antimycotic activities from combined action of antimycotic lyticase and polyethyleneimine on carbon nanotubes provides a new antimycotic protection for medicine, food industry, and other biochemical processes.
在这项工作中，带正电荷的聚合物聚乙烯亚胺被用于对碳纳米管和活性炭进行功能化处理，以负载抗真菌酶溶菌酶。有趣的是，聚乙烯亚胺在使碳材料功能化方面发挥了双重作用，既能协同增强负载的溶菌酶的抗真菌活性，又能展现出其自身明显的抗真菌活性，其中负载在功能化碳纳米管上的溶菌酶的增强酶活性比溶液中游离酶的活性高2.8倍以上。负载在功能化碳纳米管上的溶菌酶的实际活性被应用，与游离溶菌酶相比，对细菌的消化裂解速度要快得多。抗真菌溶菌酶和聚乙烯亚胺在碳纳米管上的联合作用所产生的协同且强效的抗真菌活性为医药、食品工业和其他生化过程提供了一种新的抗真菌保护。
