Morales-Guadarrama Axayacatl, Salgado-Ceballos Hermelinda, Grijalva Israel, Morales-Corona Juan, Hernández-Godínez Braulio, Ibáñez-Contreras Alejandra, Ríos Camilo, Diaz-Ruiz Araceli, Cruz Guillermo Jesus, Olayo María Guadalupe, Sánchez-Torres Stephanie, Mondragón-Lozano Rodrigo, Alvarez-Mejia Laura, Fabela-Sánchez Omar, Olayo Roberto
Centro Nacional de Investigación en Imagenología e Instrumentación Médica, Universidad Autónoma Metropolitana Iztapalapa, CDMX, Mexico City 09340, Mexico.
Departamento de Ingeniería Eléctrica, Universidad Autónoma Metropolitana Iztapalapa, CDMX, Mexico City 09340, Mexico.
Polymers (Basel). 2022 Feb 28;14(5):962. doi: 10.3390/polym14050962.
In spinal cord injury (SCI) there is damage to the nervous tissue, due to the initial damage and pathophysiological processes that are triggered subsequently. There is no effective therapeutic strategy for motor functional recovery derived from the injury. Several studies have demonstrated neurons growth in cell cultures on polymers synthesized by plasma derived from pyrrole, and the increased recovery of motor function in rats by implanting the polymer in acute states of the SCI in contusion and transection models. In the process of transferring these advances towards humans it is recommended to test in mayor species, such as nonhuman primates, prioritizing the use of non-invasive techniques to evaluate the injury progression with the applied treatments. This work shows the ability of diffusion tensor imaging (DTI) to evaluate the evolution of the SCI in nonhuman primates through the fraction of anisotropy (FA) analysis and the diffusion tensor tractography (DTT) calculus. The injury progression was analysed up to 3 months after the injury day by FA and DTT. The FA recovery and the DTT re-stabilization were observed in the experimental implanted subject with the polymer, in contrast with the non-implanted subject. The parameters derived from DTI are concordant with the histology and the motor functional behaviour.
在脊髓损伤（SCI）中，由于最初的损伤以及随后引发的病理生理过程，神经组织会受到损害。目前尚无针对该损伤后运动功能恢复的有效治疗策略。多项研究表明，在由吡咯衍生的等离子体合成的聚合物上进行细胞培养时神经元会生长，并且在挫伤和横断模型中，将聚合物植入SCI急性期的大鼠体内可使运动功能恢复增强。在将这些进展转化应用于人类的过程中，建议在较大的物种（如非人灵长类动物）中进行测试，并优先使用非侵入性技术来评估所应用治疗方法对损伤进展的影响。这项工作展示了扩散张量成像（DTI）通过各向异性分数（FA）分析和扩散张量纤维束成像（DTT）计算来评估非人灵长类动物SCI进展的能力。通过FA和DTT对损伤后长达3个月的损伤进展进行了分析。与未植入的受试者相比，在植入聚合物的实验受试者中观察到了FA恢复和DTT重新稳定。DTI得出的参数与组织学和运动功能行为一致。
