Plikusiene Ieva, Maciulis Vincentas, Ramanavicius Arunas, Ramanaviciene Almira
Nanotechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania.
State Research Institute Centre for Physical Sciences and Technology, Sauletekio Ave. 3, LT-10257 Vilnius, Lithuania.
Polymers (Basel). 2022 Mar 7;14(5):1056. doi: 10.3390/polym14051056.
Polymers represent materials that are applied in almost all areas of modern life, therefore, the characterization of polymer layers using different methods is of great importance. In this review, the main attention is dedicated to the non-invasive and label-free optical and acoustic methods, namely spectroscopic ellipsometry (SE) and quartz crystal microbalance with dissipation (QCM-D). The specific advantages of these techniques applied for in situ monitoring of polymer layer formation and characterization, biomolecule immobilization, and registration of specific interactions were summarized and discussed. In addition, the exceptional benefits and future perspectives of combined spectroscopic ellipsometry and QCM-D (SE/QCM-D) in one measurement are overviewed. Recent advances in the discussed area allow us to conclude that especially significant breakthroughs are foreseen in the complementary application of both QCM-D and SE techniques for the investigation of polymer structure and assessment of the interaction between biomolecules such as antigens and antibodies, receptors and ligands, and complementary DNA strands.
聚合物是应用于现代生活几乎所有领域的材料，因此，采用不同方法对聚合物层进行表征具有重要意义。在本综述中，主要关注非侵入性和无标记的光学和声学方法，即光谱椭偏仪（SE）和带耗散监测的石英晶体微天平（QCM-D）。总结并讨论了这些技术在聚合物层形成与表征的原位监测、生物分子固定以及特定相互作用记录方面应用的具体优势。此外，还概述了在一次测量中结合光谱椭偏仪和QCM-D（SE/QCM-D）的独特优势及未来前景。该领域的最新进展使我们能够得出结论，预计在QCM-D和SE技术的互补应用方面将取得特别重大的突破，用于研究聚合物结构以及评估生物分子之间的相互作用，如抗原与抗体、受体与配体以及互补DNA链之间的相互作用。
