Nakagawa K, Obara T, Matsubara M, Horikawa H
Endocrinol Jpn. 1978 Apr;25(2):197-9. doi: 10.1507/endocrj1954.25.197.
An enzyme-linked immunoassay for somatostatin using somatostatin-alkaline phosphatase conjugate as "labeled" antigen was developed. Minimal detectable dose at present was 40 pg per tube. Serial dilutions of rat hypothalamic extract gave a gradual change of antibody-bound alkaline phosphatase activity which was parallel to that with standard somatostatin. Precision and accuracy of the method were comparable to those in radioimmunoassay reported by others. This method will be a useful tool for the determination of somatostatin, especially in tissues.
建立了一种以生长抑素 - 碱性磷酸酶结合物作为“标记”抗原的生长抑素酶联免疫分析方法。目前每管的最低检测剂量为40皮克。大鼠下丘脑提取物的系列稀释导致抗体结合的碱性磷酸酶活性逐渐变化，这与标准生长抑素的变化平行。该方法的精密度和准确度与其他人报道的放射免疫分析相当。这种方法将成为测定生长抑素的有用工具，尤其是在组织中。
