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采用 SWATH-MS 蛋白质组学技术研究砷的神经毒性作用和香菇多糖的神经保护作用。

Investigating the Neurotoxic Impacts of Arsenic and the Neuroprotective Effects of Dictyophora Polysaccharide Using SWATH-MS-Based Proteomics.

机构信息

The Key Laboratory of Environmental Pollution Monitoring and Disease Control, School of Public Health, Ministry of Education, Guizhou Medical University, Guiyang 550025, China.

College of Life Science and Oceanography, Shenzhen University, Shenzhen 518060, China.

出版信息

Molecules. 2022 Feb 23;27(5):1495. doi: 10.3390/molecules27051495.

DOI:10.3390/molecules27051495
PMID:35268596
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8911851/
Abstract

Arsenic (As) is one of the most important toxic elements in the natural environment. Currently, although the assessment of the potential health risks of chronic arsenic poisoning has received great attention, the research on the effects of arsenic on the brain is still limited. It has been reported that dictyophora polysaccharide (DIP), a common bioactive natural compound found in dietary plants, could reduce arsenic toxicity. Following behavioral research, comparative proteomics was performed to explore the molecular mechanism of arsenic toxicity to the hippocampi of SD (Sprague Dawley) rats and the protective effect of DIP. The results showed that exposure to arsenic impaired the spatial learning and memory ability of SD rats, while DIP treatment improved both the arsenic-exposed rats. Proteomic analysis showed that arsenic exposure dysregulated the expression of energy metabolism, apoptosis, synapse, neuron, and mitochondria related proteins in the hippocampi of arsenic-exposed rats. However, DIP treatment reversed or restored the expression levels of these proteins, thereby improving the spatial learning and memory ability of arsenic-exposed rats. This study is the first to use high-throughput proteomics to reveal the mechanism of arsenic neurotoxicity in rats as well as the protective mechanism of DIP against arsenic neurotoxicity.

摘要

砷（As）是自然环境中最重要的有毒元素之一。目前，尽管慢性砷中毒的潜在健康风险评估受到了极大关注，但砷对大脑影响的研究仍很有限。有报道称，香菇多糖（DIP）是一种常见的膳食植物中含有的生物活性天然化合物，可降低砷毒性。在进行行为研究之后，进行了比较蛋白质组学研究，以探讨砷对 SD（Sprague Dawley）大鼠海马的毒性作用以及 DIP 的保护作用的分子机制。结果表明，砷暴露会损害 SD 大鼠的空间学习和记忆能力，而 DIP 处理则改善了暴露于砷的大鼠的这两种能力。蛋白质组学分析表明，砷暴露会使砷暴露大鼠海马中的能量代谢、细胞凋亡、突触、神经元和线粒体相关蛋白的表达失调。然而，DIP 处理可逆转或恢复这些蛋白质的表达水平，从而改善暴露于砷的大鼠的空间学习和记忆能力。本研究首次使用高通量蛋白质组学技术揭示了砷对大鼠神经毒性的作用机制以及 DIP 对砷神经毒性的保护作用机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/ed44af4ef4f0/molecules-27-01495-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/15d090519f38/molecules-27-01495-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/4cc339508651/molecules-27-01495-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/7d7dc97497fd/molecules-27-01495-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/e3ec9297a693/molecules-27-01495-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/e48c550b8e60/molecules-27-01495-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/e085abe7946c/molecules-27-01495-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/608bee4c11c9/molecules-27-01495-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/2cfc33cae5dc/molecules-27-01495-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/ed44af4ef4f0/molecules-27-01495-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/15d090519f38/molecules-27-01495-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/4cc339508651/molecules-27-01495-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/7d7dc97497fd/molecules-27-01495-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/e3ec9297a693/molecules-27-01495-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/e48c550b8e60/molecules-27-01495-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/e085abe7946c/molecules-27-01495-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/608bee4c11c9/molecules-27-01495-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/2cfc33cae5dc/molecules-27-01495-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/833d/8911851/ed44af4ef4f0/molecules-27-01495-g009.jpg

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