Ku Jeong-Kui, Kim Il-Hyung, Shim Jung Hee, Kim Yu Ha, Kim Baek Hyun, Kim Young-Kyun, Yun Pil-Young
Department of Oral and Maxillofacial Surgery, Gangnam Severance Hospital, Yonsei University Health System, Seoul 06273, Korea.
Department of Oral and Maxillofacial Surgery, Armed Forces Capital Hospital, Armed Forces Medical Command, Seongnam-si 13574, Korea.
Materials (Basel). 2022 Mar 4;15(5):1921. doi: 10.3390/ma15051921.
This study aimed to evaluate the biomechanical properties in vitro and the bone regeneration of whitlockite (WH) compared with hydroxyapatite (HA) or β-tricalcium phosphate (β-TCP)-based material. We investigated the morphology and phase composition of the bone grafts using a scanning electron microscope and X-ray diffractometer patterns and tested the compressive strength. Four circular defects of 8 mm in diameter were created on the calvaria of twelve rabbits. One defect was left empty, and each of the other defects was filled with WH, HA, and β-TCP. At 4 and 8 weeks, the specimens were harvested to evaluate for the new bone formation and the remaining bone grafts. Regarding the biomechanical properties, the three grafts had a similar micropore size, and WH showed nanopores. The compressive strength of WH was higher than HA and β-TCP without statistical significance. The radiological and histomorphometric analyses demonstrated that the new bone formation was similar among the groups. The remaining bone graft of the WH group was greater than that of the HA and β-TCP groups at 4 weeks (p < 0.05), and the total bone area of the WH, HA, and β-TCP groups was greater than that of the other (p < 0.01). WH has excellent volumetric stability and osteoconductivity compared with HA and β-TCP.
本研究旨在评估与羟基磷灰石（HA）或β-磷酸三钙（β-TCP）基材料相比，白磷钙石（WH）的体外生物力学性能和骨再生情况。我们使用扫描电子显微镜和X射线衍射图谱研究了骨移植材料的形态和相组成，并测试了抗压强度。在12只兔子的颅骨上制造了4个直径为8mm的圆形缺损。一个缺损保持为空，其他每个缺损分别填充WH、HA和β-TCP。在4周和8周时，采集标本以评估新骨形成和剩余的骨移植材料。关于生物力学性能，三种移植材料具有相似的微孔尺寸，而WH显示出纳米孔。WH的抗压强度高于HA和β-TCP，但无统计学意义。放射学和组织形态计量学分析表明，各组之间的新骨形成相似。在4周时，WH组剩余的骨移植材料大于HA组和β-TCP组（p<0.05），且WH组、HA组和β-TCP组的总骨面积大于其他组（p<0.01）。与HA和β-TCP相比，WH具有优异的体积稳定性和骨传导性。
