Yang Cheryl, Unursaikhan Otgonbayar, Lee Jung-Seok, Jung Ui-Won, Kim Chang-Sung, Choi Seong-Ho
Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul, 120-752, Republic of Korea.
J Biomed Mater Res B Appl Biomater. 2014 Jan;102(1):80-8. doi: 10.1002/jbm.b.32984. Epub 2013 Jul 13.
Various synthetic bone substitutes have been developed to reconstruct the bony defects that clinicians often encounter during surgical procedures. Among various synthetic bone substitutes, calcium phosphate (Ca-P) ceramics have been investigated because their composition and structure are similar to those of human bone. We evaluated the bone healing and biodegradation patterns of three types of Ca-P ceramic particle with various hydroxyapatite (HA)/β-tricalcium phosphate (β-TCP) weight ratio: pure β-TCP, biphasic Ca-P (BCP) with a HA/β-TCP weight ratio of 60/40 (BCP 60/40), and BCP with an HA/β-TCP weight ratio of 20/80 (BCP 20/80). Four 8-mm-diameter defects were created in ten rabbits. Three of the defects in each rabbit were separately and randomly filled with one of the three experimental Ca-P ceramic particles, and the fourth was filled with blood clots (control). The specimens were harvested at 2 and 8 weeks post-surgery. The histologic and histometric findings revealed that the augmented space and new bone formation were significantly better for all three Ca-P ceramics than for the control group at both 2 and 8 weeks (p < 0.05). Compared to the pure β-TCP, the two BCP groups were found to provide a larger amount of newly formed bone and bone density at the 2- and 8-week post-operative periods (p < 0.05). Throughout the observation period, BCP 60/40 and BCP 20/80 exhibited a similar bone healing and biodegradation patterns with regard to both individual particles and the total augmented area in vivo.
人们已研发出各种合成骨替代物，用于修复临床医生在手术过程中经常遇到的骨缺损。在各种合成骨替代物中，磷酸钙（Ca-P）陶瓷因其成分和结构与人体骨骼相似而受到研究。我们评估了三种不同羟基磷灰石（HA）/β-磷酸三钙（β-TCP）重量比的Ca-P陶瓷颗粒的骨愈合和生物降解模式：纯β-TCP、HA/β-TCP重量比为60/40的双相Ca-P（BCP 60/40）以及HA/β-TCP重量比为20/80的BCP（BCP 20/80）。在十只兔子身上制造了四个直径为8毫米的骨缺损。每只兔子的三个缺损分别随机填充三种实验性Ca-P陶瓷颗粒中的一种，第四个缺损填充血凝块（对照组）。在术后2周和8周采集标本。组织学和组织计量学结果显示，在2周和8周时，所有三种Ca-P陶瓷的骨缺损增大空间和新骨形成均显著优于对照组（p < 0.05）。与纯β-TCP相比，两个BCP组在术后2周和8周时新形成的骨量和骨密度更大（p < 0.05）。在整个观察期内，就体内单个颗粒和总的骨缺损增大区域而言，BCP 60/40和BCP 20/80表现出相似的骨愈合和生物降解模式。
