Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia, ES-46022 Valencia, Spain.
Plant Production Department, Universitat Politècnica de València, ES-46022 Valencia, Spain.
Cells. 2022 Feb 24;11(5):795. doi: 10.3390/cells11050795.
To face the challenges of climate change and sustainable food production, it is essential to develop crop genome editing techniques to pinpoint key genes involved in abiotic stress signaling. The identification of those prevailing abscisic acid (ABA) receptors that mediate plant-environment interactions is quite challenging in polyploid plants because of the high number of genes in the PYR/PYL/RCAR ABA receptor family. is a biotechnological crop amenable to genome editing, and given the importance of ABA signaling in coping with drought stress, we initiated the analysis of its 23-member family of ABA receptors through multiplex CRISPR/Cas9-mediated editing. We generated several high-order mutants impaired in and receptors, which showed certain insensitivity to ABA for inhibition of seedling establishment, growth, and development of shoot and lateral roots as well as reduced sensitivity to the PYL1-agonist cyanabactin (CB). However, in these high-order mutants, regulation of transpiration was not affected and was responsive to ABA treatment. This reveals a robust and redundant control of transpiration in this allotetraploid plant that probably reflects its origin from the extreme habitat of central Australia.
为了应对气候变化和可持续粮食生产的挑战，开发作物基因组编辑技术以精确定位参与非生物胁迫信号的关键基因至关重要。由于 PYR/PYL/RCAR ABA 受体家族中的基因数量众多，因此在多倍体植物中鉴定那些普遍存在的调节植物-环境相互作用的脱落酸（ABA）受体是相当具有挑战性的。拟南芥是一种可进行基因组编辑的生物技术作物，鉴于 ABA 信号在应对干旱胁迫方面的重要性，我们开始通过多重 CRISPR/Cas9 介导的编辑分析其 23 个成员的 ABA 受体家族。我们生成了多个在 和 受体中受损的高级突变体，这些突变体对 ABA 抑制幼苗建立、生长和发育以及侧根的生长表现出一定的不敏感性，并且对 PYL1 激动剂氰钴胺素（CB）的敏感性降低。然而，在这些高级突变体中，蒸腾作用的调节不受影响且对 ABA 处理有响应。这揭示了这种同源四倍体植物中蒸腾作用的强大和冗余控制，这可能反映了它起源于澳大利亚中部的极端生境。
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