Vogl A W, Grove B D, Lew G J
Anat Rec. 1986 Aug;215(4):331-41. doi: 10.1002/ar.1092150402.
We have investigated the possibility that the complex patterns of fluorescence associated with spermatids of the ground squirrel labeled with 7-nitrobenz-2-oxa-1,3-diazole-phallacidin (NBD-phallacidin) are due to the presence of filamentous actin within the spermatids themselves rather than to actin in attached Sertoli cell ectoplasmic specializations, as previously reported (J. Cell Biol., 100:814-825). Enzymatic treatments (trypsin, DNAase 1) freed Sertoli cell ectoplasmic specializations from spermatids and resulted in a loss, from the spermatids, of the complex fluorescence patterns, suggesting that the latter were generated by labeled actin in ectoplasmic specializations. Moreover, ectoplasmic specializations that were detached enzymatically from spermatids demonstrated the same fluorescence patterns as those emitted from spermatids in the intact or mechanically fragmented seminiferous epithelium. Most spermatids, however, do display a weak and diffuse pattern of fluorescence that changes during spermatogenesis and that is localized between the acrosomal cap and nucleus. S-1 decoration confirmed this subacrosomal localization and further demonstrated that the actin in adjacent Sertoli cell ectoplasmic specializations is arranged in a unipolar fashion. We conclude that the complex patterns of actin fluorescence associated with mechanically isolated spermatids are a superimposition of both Sertoli cell and germ cell actin; however, the latter is either poorly detected or not detected at all when Sertoli cell ectoplasmic specializations overlie the germ cells.
我们已经研究了这样一种可能性，即与用7-硝基苯-2-恶唑-1,3-二氮杂环戊二烯-鬼笔环肽（NBD-鬼笔环肽）标记的地松鼠精子细胞相关的复杂荧光模式，是由于精子细胞本身存在丝状肌动蛋白，而不是如先前报道的那样（《细胞生物学杂志》，100:814 - 825），是由于附着的支持细胞外质特化结构中的肌动蛋白。酶处理（胰蛋白酶、DNA酶1）使支持细胞外质特化结构与精子细胞分离，并导致精子细胞失去复杂的荧光模式，这表明后者是由外质特化结构中标记的肌动蛋白产生的。此外，通过酶法从精子细胞上分离下来的外质特化结构显示出与完整或机械破碎的生精上皮中的精子细胞发出的荧光模式相同。然而，大多数精子细胞确实显示出一种微弱且弥散的荧光模式，这种模式在精子发生过程中会发生变化，并且定位在顶体帽和细胞核之间。S-1标记证实了这种顶体下定位，并进一步表明相邻支持细胞外质特化结构中的肌动蛋白以单极方式排列。我们得出结论，与机械分离的精子细胞相关的肌动蛋白荧光复杂模式是支持细胞和生殖细胞肌动蛋白的叠加；然而，当支持细胞外质特化结构覆盖生殖细胞时，后者要么很难被检测到，要么根本检测不到。
