Zhu Longming, Feng Lele, Yu Xiaomin, Fu Xujun, Yang Qinghua, Jin Hangxia, Yuan Fengjie
Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
Plants (Basel). 2022 Feb 28;11(5):657. doi: 10.3390/plants11050657.
Anthracnose caused by is a major fungal disease of soybean, especially vegetable soybean (edamame). Studies of this disease have mainly focused on resistance evaluation, but the primary methods used-in vivo inoculation of pods or plants under greenhouse or field conditions-have limitations with respect to accuracy, stability, scale, and environmental safety. In this study, we developed a method for inoculating pods in vitro by soaking in a mycelial suspension. We optimized the crucial components, including the mycelial suspension concentration (40 to 60 mg mL), the maturity of the sampled pods (15 days after flowering), and the post-inoculation incubation period (5 days). Application of the mycelial suspension by soaking rather than spraying improved the efficiency of inoculation and made large-scale evaluation possible. Using this method, we evaluated 589 soybean germplasm resources (275 cultivars, 233 landraces, and 81 wild accessions). We identified 25 highly resistant cultivars, 11 highly resistant landraces, but only one highly resistant wild accession. Our results will aid future research on soybean anthracnose resistance, including gene discovery, the elucidation of molecular mechanisms, and the breeding of resistant cultivars.
由[病原体名称未给出]引起的炭疽病是大豆尤其是鲜食大豆（毛豆）的一种主要真菌病害。对这种病害的研究主要集中在抗性评估方面，但所采用的主要方法——在温室或田间条件下对豆荚或植株进行活体接种——在准确性、稳定性、规模和环境安全性方面存在局限性。在本研究中，我们开发了一种通过在菌丝悬浮液中浸泡来对豆荚进行体外接种的方法。我们优化了关键要素，包括菌丝悬浮液浓度（40至60毫克/毫升）、采样豆荚的成熟度（开花后15天）以及接种后的培养期（5天）。通过浸泡而非喷雾施用菌丝悬浮液提高了接种效率并使大规模评估成为可能。利用这种方法，我们评估了589份大豆种质资源（275个栽培品种、233个地方品种和81份野生种质）。我们鉴定出25个高抗栽培品种、11个高抗地方品种，但只有1份高抗野生种质。我们的结果将有助于未来大豆炭疽病抗性研究，包括基因发现、分子机制阐明以及抗病品种的培育。
