Yang Hui-Ching, Hartman Glen L
Department of Natural Resources and Environmental Sciences.
United States Department of Agriculture-Agricultural Research Service and Department of Crop Sciences, University of Illinois, Urbana 61801.
Plant Dis. 2015 Jan;99(1):143-148. doi: 10.1094/PDIS-03-14-0228-RE.
Anthracnose of soybean (Glycine max) occurs throughout the soybean production areas of the world. There is little information on evaluating inoculation techniques or evaluating soybean germplasm for resistance. The objectives of this study were to develop a reliable inoculation technique for evaluating soybean for resistance to Colletotrichum truncatum and to evaluate soybean genotypes for resistance. Inoculated plants incubated in a dew chamber for 48 or 72 h had higher (P = 0.05) area under disease progress curve (AUDPC) values than when incubated for 24 h. Three experiments evaluated soybean genotypes for resistance to C. truncatum. In the first experiment using 15 soybean genotypes, 'Mandarin' had lower (P = 0.05) AUDPC values than all the other genotypes except for 'Mandarin 507', 'Mandarin (Ottawa)', and 'Boone'. In the second experiment using 28 soybean genotypes, Mandarin 507 had lower (P = 0.05) AUDPC values compared with all other soybean genotypes except 'Early White Eyebrow', 'Mandarin Yowa', Boone, and 'Manchuria'. In the third experiment, Mandarin 507 had lower (P = 0.05) AUDPC values compared with 23 other soybean genotypes except 'Spry', Mandarin, and 'Iroquois'. Plants of Mandarin 507 and 'Williams 82' were inoculated at the vegetative and reproductive growth stages; Mandarin 507 had lower (P = 0.05) AUDPC values compared with Williams 82 for both growth stages evaluated, and Mandarin 507 had less (P = 0.05) pod area covered by lesions compared with the pods of Williams 82. This study provided a descriptive method to inoculate soybean plants with C. truncatum and identified soybean genotypes with resistance to anthracnose.
大豆炭疽病（Glycine max）在世界大豆产区均有发生。关于评估接种技术或评估大豆种质资源抗性的信息较少。本研究的目的是开发一种可靠的接种技术，用于评估大豆对截形炭疽菌的抗性，并评估大豆基因型的抗性。在露室中培养48或72小时的接种植株，其病情进展曲线下面积（AUDPC）值高于培养24小时的植株（P = 0.05）。三项实验评估了大豆基因型对截形炭疽菌的抗性。在第一项实验中，使用了15个大豆基因型，除了“Mandarin 507”、“Mandarin（渥太华）”和“Boone”外，“Mandarin”的AUDPC值低于所有其他基因型（P = 0.05）。在第二项实验中，使用了28个大豆基因型，与除“Early White Eyebrow”、“Mandarin Yowa”、Boone和“Manchuria”外的所有其他大豆基因型相比，Mandarin 507的AUDPC值较低（P = 0.05）。在第三项实验中，与除“Spry”、Mandarin和“Iroquois”外的其他23个大豆基因型相比，Mandarin 507的AUDPC值较低（P = 0.05）。对Mandarin 507和“Williams 82”的植株在营养生长和生殖生长阶段进行接种；在评估的两个生长阶段，Mandarin 507的AUDPC值均低于Williams 82（P = 0.05），并且与Williams 82的豆荚相比，Mandarin 507的病斑覆盖的豆荚面积更小（P = 0.05）。本研究提供了一种用截形炭疽菌接种大豆植株的描述性方法，并鉴定出了对炭疽病具有抗性的大豆基因型。
