Oliviero Maria, Langellotti Antonio Luca, Russo Giovanni L, Baselice Marco, Donadio Andrea, Ritieni Alberto, Graziani Giulia, Masi Paolo
CAISIAL Center, University of Naples Federico II, 80055 Portici, Italy.
Unit of Food Science and Technology, Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy.
Plants (Basel). 2022 Mar 5;11(5):701. doi: 10.3390/plants11050701.
(Asteraceae family) is a perennial plant native to Mediterranean regions. This plant represents a source of high-value compounds, such as polyphenols and fatty acids that have several industrial applications. However, in vitro plant cell cultures can represent a valid alternative to in-field cultivation and facilitate the extraction of metabolites of commercial interest. Generally, sucrose is the main sugar used for plant cell cultures, but other carbon sources can be considered. Here, we investigated the potential use of alternative organic carbon sources, such as galactose, maltose, glucose, glycerol, fructose, lactose, and starch, for the cultivation of cells. Moreover, cardoon cells were collected, and an extraction of polyphenols and oils was performed to study the effects of different carbon sources on the production of bioactive molecules. This study provided evidence that cardoon cell growth can be supported by carbon sources other than sucrose. However, the carbon source inducing optimum growth, did not necessarily induce the highest production of high-value compounds.
（菊科）是一种原产于地中海地区的多年生植物。这种植物是高价值化合物的来源，如具有多种工业应用的多酚和脂肪酸。然而，植物细胞体外培养可以成为田间种植的有效替代方法，并有助于提取具有商业价值的代谢物。一般来说，蔗糖是植物细胞培养中使用的主要糖类，但也可以考虑其他碳源。在这里，我们研究了替代有机碳源，如半乳糖、麦芽糖、葡萄糖、甘油、果糖、乳糖和淀粉，用于细胞培养的潜在用途。此外，收集了刺菜蓟细胞，并进行了多酚和油的提取，以研究不同碳源对生物活性分子生产的影响。这项研究提供了证据，表明除蔗糖外的其他碳源可以支持刺菜蓟细胞的生长。然而，诱导最佳生长的碳源不一定能诱导高价值化合物的最高产量。
