Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou, China.
College of Life Science and Engineering, Northwest Minzu University, Lanzhou, China.
PLoS One. 2022 Mar 10;17(3):e0261710. doi: 10.1371/journal.pone.0261710. eCollection 2022.
Apocynum venetum L. (Apocynaceae) is valuable for its medicinal compounds and fiber content. Native A. venetum populations are threatened and require protection. Wild A. venetum resources are limited relative to market demand and a poor understanding of the composition of A. venetum at the molecular level. The chloroplast genome contains genetic markers for phylogenetic analysis, genetic diversity evaluation, and molecular identification. In this study, the entire genome of the A. venetum chloroplast was sequenced and analyzed. The A. venetum cp genome is 150,878 bp, with a pair of inverted repeat regions (IRA and IRB). Each inverted repeat region is 25,810 bp, which consist of large (LSC, 81,951 bp) and small (SSC, 17,307 bp) single copy areas. The genome-wide GC content was 38.35%, LSC made up 36.49%, SSC made up 32.41%, and IR made up 43.3%. The A. venetum chloroplast genome encodes 131 genes, including 86 protein-coding genes, eight ribosomal RNA genes, and 37 transfer RNA genes. This study identified the unique characteristics of the A. venetum chloroplast genome, which will help formulate effective conservation and management strategies as well as molecular identification approaches for this important medicinal plant.
罗布麻（夹竹桃科）以其药用化合物和纤维含量而具有价值。本地罗布麻种群受到威胁，需要保护。与市场需求相比，野生罗布麻资源有限，而且对罗布麻的分子水平组成了解不足。叶绿体基因组包含用于系统发育分析、遗传多样性评估和分子鉴定的遗传标记。在这项研究中，测序和分析了罗布麻叶绿体的整个基因组。罗布麻 cp 基因组长 150878bp，具有一对反向重复区域（IRA 和 IRB）。每个反向重复区域长 25810bp，由大（LSC，81951bp）和小（SSC，17307bp）单拷贝区组成。基因组的 GC 含量为 38.35%，LSC 占 36.49%，SSC 占 32.41%，IR 占 43.3%。罗布麻叶绿体基因组编码 131 个基因，包括 86 个蛋白编码基因、8 个核糖体 RNA 基因和 37 个转移 RNA 基因。本研究鉴定了罗布麻叶绿体基因组的独特特征，这将有助于制定有效的保护和管理策略，以及对这种重要药用植物进行分子鉴定的方法。
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