对[具体植物名称1]和[具体植物名称2]进行基因组调查、转录组和代谢组分析以揭示主要黄酮类生物合成途径。

Genomic Survey, Transcriptome, and Metabolome Analysis of and to Reveal Major Flavonoid Biosynthesis Pathways.

作者信息

Gao Gang, Chen Ping, Chen Jikang, Chen Kunmei, Wang Xiaofei, Abubakar Aminu Shehu, Liu Ning, Yu Chunming, Zhu Aiguo

机构信息

Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China.

出版信息

Metabolites. 2019 Dec 5;9(12):296. doi: 10.3390/metabo9120296.

plants, especially and are rich in flavonoids. In the present study, a whole genome survey of the two species was initially carried out to optimize the flavonoid biosynthesis-correlated gene mining. Then, the metabolome and transcriptome analyses were combined to elucidate the flavonoid biosynthesis pathways. Both species have small genome sizes of 232.80 Mb () and 233.74 Mb () and showed similar metabolite profiles with flavonols being the main differentiated flavonoids between the two specie. Positive correlation of gene expression levels (flavonone-3 hydroxylase, anthocyanidin reductase, and flavonoid 3-O-glucosyltransferase) and total flavonoid content were observed. The contents of quercitrin, hyperoside, and total anthocyanin in were found to be much higher than in and such was thought to be the reason for the morphological difference in color of and . This study provides valuable genomic and metabolome information for understanding of and , and lays a foundation for elucidating genus plant flavonoid biosynthesis.

植物，尤其是[具体植物名称1]和[具体植物名称2]富含黄酮类化合物。在本研究中，首先对这两个物种进行了全基因组调查，以优化与黄酮类生物合成相关的基因挖掘。然后，结合代谢组和转录组分析来阐明黄酮类生物合成途径。这两个物种的基因组都较小，分别为232.80 Mb（[具体植物名称1]）和233.74 Mb（[具体植物名称2]），并且显示出相似的代谢物谱，黄酮醇是这两个物种之间主要的差异黄酮类化合物。观察到基因表达水平（黄酮酮-3-羟化酶、花青素还原酶和黄酮类3-O-葡萄糖基转移酶）与总黄酮含量呈正相关。发现[具体植物名称1]中槲皮苷、金丝桃苷和总花青素的含量远高于[具体植物名称2]，这被认为是[具体植物名称1]和[具体植物名称2]颜色形态差异的原因。本研究为理解[具体植物名称1]和[具体植物名称2]提供了有价值的基因组和代谢组信息，并为阐明[植物属名]属植物黄酮类生物合成奠定了基础。