牛脑酸性成纤维细胞生长因子（FGF）的一级结构。

Primary structure of bovine brain acidic fibroblast growth factor (FGF).

作者信息

Esch F, Ueno N, Baird A, Hill F, Denoroy L, Ling N, Gospodarowicz D, Guillemin R

出版信息

Biochem Biophys Res Commun. 1985 Dec 17;133(2):554-62. doi: 10.1016/0006-291x(85)90942-8.

Abstract

The major anionic mitogenic polypeptide for endothelial cells, acidic fibroblast growth factor (FGF), has been purified to homogeneity from bovine brain and its complete primary structure established by gas-phase sequence analysis. The 140 amino acid (Mr 16,000) protein has been previously shown to be a potent growth factor for many diverse cell types of mesodermal origin, in vitro, and an angiogenic agent, in vivo. The amino acid sequence of bovine brain acidic FGF has a 53% absolute homology with that of bovine pituitary basic FGF suggesting that these endothelial cell mitogens are derived from a single ancestral gene.

摘要

内皮细胞的主要阴离子促有丝分裂多肽——酸性成纤维细胞生长因子（FGF），已从牛脑中纯化至同质，并通过气相序列分析确定了其完整的一级结构。这种由140个氨基酸组成（Mr 16,000）的蛋白质，先前已被证明在体外是多种中胚层来源细胞类型的有效生长因子，在体内是一种血管生成剂。牛脑酸性FGF的氨基酸序列与牛垂体碱性FGF的氨基酸序列具有53%的绝对同源性，这表明这些内皮细胞有丝分裂原源自单个祖先基因。