Hu Xiao, Yamashita Toru, Yu Haibo, Bian Zhihong, Hu Xinran, Feng Tian, Tadokoro Koh, Morihara Ryuta, Abe Koji
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan,
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Neurodegener Dis. 2021;21(5-6):117-125. doi: 10.1159/000523983. Epub 2022 Mar 10.
Alzheimer's disease (AD) is the most frequent cause of dementia among the elderly. The accumulation of amyloid beta (Aβ) and its downstream pathological events such as oxidative stress play central roles in AD. Recent studies revealed that Aβ oligomer (AβO)-induced strong neurotoxicity in SH-SY5Y cells via the induction of oxidative stress.
In the present study, we investigated the effect of two antioxidants, Tocovid and Twendee-X, on AβO-induced SH-SY5Y cell damage.
AβOs (2.5 μM) were applied to induce cellular damage in the SH-SY5Y cell line. Cell viability following AβO toxicity, Tau protein phosphorylation, cell morphology, and intracellular reactive oxygen species were assayed with or without different concentrations of Tocovid or Twendee-X.
Tocovid (60 μg/mL) and Twendee-X (150 μg/mL) significantly recovered cell viability from AβO toxicity (**p < 0.01, vs. control), attenuated Tau protein phosphorylation (**p < 0.01, vs. AβOs), improved cell morphology (**p < 0.01, vs. AβOs), and suppressed intracellular ROS (**p < 0.01, vs. AβOs) in SH-SY5Y cells.
These findings suggest the neuroprotective and therapeutic potential of Tocovid and Twendee-X for AD treatment.
阿尔茨海默病（AD）是老年人痴呆最常见的病因。淀粉样β蛋白（Aβ）的积累及其下游病理事件如氧化应激在AD中起核心作用。最近的研究表明，Aβ寡聚体（AβO）通过诱导氧化应激在SH-SY5Y细胞中诱导强烈的神经毒性。
在本研究中，我们研究了两种抗氧化剂生育三烯酚（Tocovid）和Twendee-X对AβO诱导的SH-SY5Y细胞损伤的影响。
应用AβO（2.5μM）诱导SH-SY5Y细胞系的细胞损伤。在有或没有不同浓度的Tocovid或Twendee-X的情况下，检测AβO毒性后的细胞活力、Tau蛋白磷酸化、细胞形态和细胞内活性氧。
Tocovid（60μg/mL）和Twendee-X（150μg/mL）显著恢复了AβO毒性导致的细胞活力（**p<0.01，与对照组相比），减弱了Tau蛋白磷酸化（**p<0.01，与AβO组相比），改善了细胞形态（**p<0.01，与AβO组相比），并抑制了SH-SY5Y细胞内的活性氧（**p<0.01，与AβO组相比）。
这些发现表明Tocovid和Twendee-X在AD治疗中具有神经保护和治疗潜力。
