Brussels IVF, Center for Reproductive Medicine, Vrije Universiteit Brussel, Universitair Ziekenhuis Brussel, Brussels, Belgium.
Reproduction and Immunology, Vrije Universiteit Brussel, Brussels, Belgium.
Fertil Steril. 2022 Apr;117(4):771-780. doi: 10.1016/j.fertnstert.2021.12.032. Epub 2022 Jan 1.
To study the presence of viral RNA in the follicular fluid, cumulus cells, and endometrial tissue samples in SARS-CoV-2-positive women undergoing assisted reproductive technology (ART).
Prospective, single-center, observational study.
Tertiary hospital.
PATIENT(S): A total of 16 patients undergoing transvaginal oocyte retrieval who had a positive SARS-CoV-2 RNA test <48 hours before the procedure. All patients underwent the retrieval between September 2020 and June 2021 and used in vitro fertilization or intracytoplasmic sperm injection. All embryos were vitrified to avoid conception during SARS-CoV-2 infection.
INTERVENTION(S): Follicular fluid aspirated during oocyte retrieval, cumulus cells, and endometrial samples were analyzed for SARS-CoV-2 RNA using the RealStar SARS-CoV-2 RT-PCR-Kit1.0.
MAIN OUTCOME MEASURE(S): The primary outcome parameter was the detection of viral RNA in the follicular fluid, cumulus cells, and endometrial cells. Fertilization rate, embryo developmental potential, and clinical outcome after frozen embryo transfer were secondary outcome parameters.
RESULT(S): Samples from 16 patients were analyzed. Cycle threshold values of <40 were considered positive. All samples were negative for SARS-CoV-2 viral RNA. No inflammatory lesions of the endometrium were identified histologically. Fertilization rate, embryo development, and clinical outcomes after embryo transfer were reassuring.
CONCLUSION(S): In women infected with SARS-CoV-2 who underwent ART, viral RNA was undetectable in the follicular fluid, cumulus cells, and endometrium. Caution is warranted in view of the small sample size, and the risk of SARS-CoV-2 affecting the embryo via ART cannot be ruled out. Adequate counseling of women and couples undergoing ART is crucial in parallel with further research on the effect of exposure of the early human embryo to SARS-CoV-2.
NCT04425317.
研究 SARS-CoV-2 阳性行辅助生殖技术（ART）的妇女的卵泡液、卵丘细胞和子宫内膜组织样本中是否存在病毒 RNA。
前瞻性、单中心、观察性研究。
三级医院。
共 16 例行经阴道取卵的患者，她们在手术前 48 小时内 SARS-CoV-2 RNA 检测呈阳性。所有患者均于 2020 年 9 月至 2021 年 6 月期间接受了体外受精或胞浆内精子注射。所有胚胎均被玻璃化以避免在 SARS-CoV-2 感染期间受孕。
在取卵过程中抽吸卵泡液，使用 RealStar SARS-CoV-2 RT-PCR-Kit1.0 分析卵丘细胞和子宫内膜样本中的 SARS-CoV-2 RNA。
主要结局参数是检测卵泡液、卵丘细胞和子宫内膜细胞中的病毒 RNA。受精率、胚胎发育潜能和冷冻胚胎移植后的临床结局是次要结局参数。
分析了 16 名患者的样本。循环阈值<40 被认为是阳性。所有样本均未检测到 SARS-CoV-2 病毒 RNA。组织学上未发现子宫内膜的炎症病变。受精率、胚胎发育和胚胎移植后的临床结局令人放心。
在感染 SARS-CoV-2 行 ART 的妇女中，卵泡液、卵丘细胞和子宫内膜中均无法检测到病毒 RNA。鉴于样本量小，需要谨慎，并不能排除 SARS-CoV-2 通过 ART 影响胚胎的风险。对接受 ART 的妇女和夫妇进行充分的咨询至关重要，同时还需要进一步研究早期人类胚胎暴露于 SARS-CoV-2 的影响。
NCT04425317。
