Vilas-Arrondo Nair, Gomes-Dos-Santos André, Pérez Montse, Baldó Francisco, Veríssimo Ana, Catarino Diana, Machado André M, Román-Marcote Esther, Bañón Rafael, Froufe Elsa, Castro L Filipe C
AQUACOV, Instituto Español de Oceanografía (IEO, CSIC), Centro Oceanográfico de Vigo, Vigo, Spain.
UVIGO, PhD Program "Marine Science, Technology and Management" (DoMAR), Faculty of Biology, University of Vigo, Vigo, Spain.
Mitochondrial DNA B Resour. 2022 Mar 2;7(3):434-437. doi: 10.1080/23802359.2022.2044403. eCollection 2022.
Chondrichthyans (sharks, rays and chimeras) are a fascinating and highly vulnerable group of early branching gnathostomes. However, they remain comparatively poorly sampled from the point of view of molecular resources, with deep water taxa being particularly data deficient. The development of long-read sequencing technologies enables the analysis of phylogenetic relationships through a precise and reliable assembly of complete mtDNA genomes. The sequencing and characterization of the complete mitogenome of the opal chimera Luchetti, Iglésias et Sellos 2011, using the long-read technique PacBio HiFi is presented. The entire mitogenome was 23,411 bp long and shows the same overall content, i.e. 13 protein-coding genes, 22 transfer RNA and 2 ribosomal RNA genes, as all other examined Chondrichthyan mitogenomes. Phylogenetic reconstructions using all available Chondrichthyan mitogenomes, including 11 Holocephali (chimeras and ratfishes), places within the Chimaeridae family. Furthermore, the results reinforce previous findings, showing the genus as paraphyletic and thus highlighting the need to expand molecular approaches in this group of cartilaginous fishes.
软骨鱼类（鲨鱼、鳐鱼和银鲛）是早期分支有颌类中一个迷人且高度脆弱的类群。然而，从分子资源的角度来看，它们的样本仍然相对较少，深水类群的数据尤其匮乏。长读长测序技术的发展使得通过精确可靠地组装完整的线粒体DNA基因组来分析系统发育关系成为可能。本文介绍了利用长读长技术PacBio HiFi对猫眼银鲛（Luchetti, Iglésias et Sellos 2011）完整线粒体基因组的测序和特征分析。整个线粒体基因组长度为23411 bp，与所有其他已检测的软骨鱼类线粒体基因组一样，具有相同的总体组成，即13个蛋白质编码基因、22个转运RNA和2个核糖体RNA基因。使用所有可用的软骨鱼类线粒体基因组（包括11种全头亚纲鱼类（银鲛和银鲛目鱼类））进行的系统发育重建将[具体物种名称]置于银鲛科内。此外，结果强化了先前的发现，表明[属名]是并系的，从而突出了在这组软骨鱼类中扩展分子方法的必要性。
