Gambari Laura, Barone Marli, Amore Emanuela, Grigolo Brunella, Filardo Giuseppe, Iori Renato, Citi Valentina, Calderone Vincenzo, Grassi Francesco
Laboratorio RAMSES, IRCCS Istituto Ortopedico Rizzoli, Via di Barbiano 1/10, 40136 Bologna, Italy.
Applied and Translational Research Center (ATRc), IRCCS Istituto Ortopedico Rizzoli, Via di Barbiano 1/10, 40136 Bologna, Italy.
Nutrients. 2022 Jan 19;14(3):435. doi: 10.3390/nu14030435.
Osteopenia and osteoporosis are among the most prevalent consequences of ageing, urging the promotion of healthy nutritional habits as a tool in preventing bone fractures. Glucosinolates (GLSs) are organosulfur compounds considered relatively inert precursors of reactive derivatives isothiocyanates (ITCs). Recent evidence suggests that GLSs may exert biological properties based on their capacity to release hydrogen sulfide (HS). HS-donors are known to exert anabolic function on bone cells. Here, we investigated whether a GLS, glucoraphanin (GRA) obtained from Tuscan black kale, promotes osteogenesis in human mesenchymal stromal cells (hMSCs). HS release in buffer and intracellular HS levels were detected by amperometric measurements and fluorimetric/cytofluorimetric analyses, respectively. Alizarin red staining assay and real-time PCR were performed to evaluate mineral apposition and mRNA expression of osteogenic genes. Using an in vitro cell culture model, our data demonstrate a sulforaphane (SFN)-independent osteogenic stimulation of GRA in hMSCs, at least partially attributable to HS release. In particular, GRA upregulated the expression of osteogenic genes and enhanced mineral apposition while increasing intracellular concentrations of HS. Overall, this study suggests the feasibility of using cruciferous derivatives as natural alternatives to chemical HS-donors as adjuvant therapies in the treatment of bone-wasting diseases.
骨质减少和骨质疏松是衰老最常见的后果之一，这促使人们推广健康的营养习惯，以此作为预防骨折的一种手段。硫代葡萄糖苷（GLSs）是有机硫化合物，被认为是活性衍生物异硫氰酸盐（ITCs）的相对惰性前体。最近的证据表明，GLSs可能基于其释放硫化氢（HS）的能力发挥生物学特性。已知HS供体对骨细胞具有合成代谢功能。在此，我们研究了从托斯卡纳黑甘蓝中提取的一种GLS——萝卜硫苷（GRA）是否能促进人间充质基质细胞（hMSCs）的成骨作用。分别通过安培测量法和荧光/细胞荧光分析检测缓冲液中的HS释放和细胞内HS水平。进行茜素红染色试验和实时PCR以评估成骨基因的矿物质沉积和mRNA表达。利用体外细胞培养模型，我们的数据表明，GRA在hMSCs中对成骨的刺激作用不依赖于萝卜硫素（SFN），至少部分归因于HS的释放。特别是，GRA上调了成骨基因的表达，增强了矿物质沉积，同时增加了细胞内HS的浓度。总体而言，本研究表明，使用十字花科衍生物作为化学HS供体的天然替代品作为辅助疗法治疗骨质流失疾病具有可行性。
