The Antioxidant Properties of Glucosinolates in Cardiac Cells Are Independent of HS Signaling.

The organic sulfur-containing compounds glucosinolates (GSLs) and the novel gasotransmitter HS are known to have cardioprotective effects. This study investigated the antioxidant effects and HS-releasing potential of three GSLs ((3)-4-(methylsulfanyl)but-3-enyl GSL or glucoraphasatin, 4-hydroxybenzyl GSL or glucosinalbin, and ()-6-(methylsulfinyl)hexyl GSL or glucohesperin) in rat cardiac cells. It was found that all three GSLs had no effect on cardiac cell viability but were able to protect against HO-induced oxidative stress and cell death. NaHS, a HS donor, also protected the cells from HO-stimulated oxidative stress and cell death. The GSLs alone or mixed with cysteine, -acetylcysteine, glutathione, HO, iron and pyridoxal-5'-phosphate, or mouse liver lysates did not induce HS release. The addition of GSLs also did not alter endogenous HS levels in cardiac cells. HO significantly induced cysteine oxidation in the cystathionine gamma-lyase (CSE) protein and inhibited the HS production rate. In conclusion, this study found that the three tested GSLs protect cardiomyocytes from oxidative stress and cell death but independently of HS signaling.