Namavar F, Kaan J A, Verweij-van Vught A M, Vel W A, Bal M, Kester A D, MacLaren D M
Eur J Clin Microbiol. 1986 Jun;5(3):324-9. doi: 10.1007/BF02017790.
Bactericidal action of human polymorphonuclear leucocytes on Escherichia coli in the presence of Bacteroides fragilis grown in subinhibitory concentrations of clindamycin, metronidazole and fusidic acid was studied. Bacteroides fragilis grown in the absence of drugs significantly inhibited the killing of Escherichia coli. Bacteroides fragilis grown in the presence of the drugs had a reduced inhibitory effect on the killing of Escherichia coli but this reduction was only significant for Bacteroides fragilis grown in 1/2 MIC of clindamycin. The phagocytosis of Bacteroides fragilis grown with and without clindamycin, as measured by killing, was the same. Complement consumption of Bacteroides fragilis grown with and without clindamycin did not differ. Clindamycin-treated Bacteroides fragilis fixed C3 to a significantly lower degree than did untreated bacteria. The chemiluminescence of Escherichia coli opsonized with serum preincubated with clindamycin-treated Bacteroides fragilis was significantly higher than with serum preincubated with untreated bacteria. These results suggested that in killing experiments of mixed Escherichia coli and Bacteroides fragilis, the mechanism underlying the reduced inhibitory capacity of clindamycin-exposed Bacteroides fragilis is related to greater availability of C3 in serum for opsonization of Escherichia coli.
研究了在脆弱拟杆菌于亚抑菌浓度的克林霉素、甲硝唑和夫西地酸中生长的情况下，人多形核白细胞对大肠杆菌的杀菌作用。在无药物条件下生长的脆弱拟杆菌显著抑制了大肠杆菌的杀灭。在有药物存在的情况下生长的脆弱拟杆菌对大肠杆菌杀灭的抑制作用有所降低，但这种降低仅在脆弱拟杆菌于1/2 MIC克林霉素中生长时才具有显著性。通过杀灭来衡量，无论有无克林霉素，脆弱拟杆菌的吞噬作用是相同的。无论有无克林霉素，脆弱拟杆菌的补体消耗没有差异。用克林霉素处理的脆弱拟杆菌固定C3的程度明显低于未处理的细菌。用与经克林霉素处理的脆弱拟杆菌预孵育的血清调理的大肠杆菌的化学发光显著高于用与未处理细菌预孵育的血清调理的大肠杆菌。这些结果表明，在混合大肠杆菌和脆弱拟杆菌的杀灭实验中，暴露于克林霉素的脆弱拟杆菌抑制能力降低的潜在机制与血清中用于大肠杆菌调理的C3可用性增加有关。
