血清细胞外囊泡精氨酸酶 1 水平升高与 2 型糖尿病：中老年人群的横断面研究。
Elevated serum extracellular vesicle arginase 1 in type 2 diabetes mellitus: a cross-sectional study in middle-aged and elderly population.
机构信息
Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Disease, No. 2 Anzhen Road, Beijing, 100029, China.
Department of Emergency, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.
出版信息
BMC Endocr Disord. 2022 Mar 11;22(1):62. doi: 10.1186/s12902-022-00982-z.
BACKGROUND
Serum extracellular vesicle (EV)-derived arginase 1 (ARG 1) plays a critical role in diabetes-associated endothelial dysfunction. This study was performed to determine the levels of serum EV-derived ARG 1 in T2DM and non-T2DM participants and to examine the association of serum EV-derived ARG 1 with T2DM incidence.
METHODS
We performed a cross-sectional study in 103 Chinese, including 73 T2DM patients and 30 non-T2DM. Serum EVs were prepared via ultracentrifugation. Serum EV-derived ARG 1 levels were measured by enzyme-linked immunosorbent assay. The correlations between serum EV-derived ARG 1 and clinical variables were analyzed. The association of serum EV-derived ARG 1 levels with T2DM was determined by multivariate logistic regression analysis. Interaction subgroup analysis was used to evaluate the interaction of the relevant baselines on the association between serum EV-derived ARG 1 levels and T2DM.
RESULTS
Serum EV-derived ARG 1 levels were significantly higher in T2DM patients compared with non-T2DM patients (p < 0.001). Correlation analysis revealed that serum EV-derived ARG 1 levels were positively associated with fasting plasma glucose (FPG) (r = 0.316, p = 0.001) and glycated hemoglobin (HbA1c) (r = 0.322, p = 0.001). Serum EV-derived ARG 1 levels were significantly associated with T2DM, especially in the subgroup of T2DM for more than 10 years (OR 1.651, 95% CI = 1.066-2.557; P value, 0.025), after adjusting for confounding factors.
CONCLUSIONS
Elevated concentration of serum EV-derived ARG 1 is closely associated with T2DM.
背景
血清细胞外囊泡（EV）衍生的精氨酸酶 1（ARG1）在糖尿病相关的内皮功能障碍中起着关键作用。本研究旨在确定 T2DM 和非 T2DM 参与者血清 EV 衍生的 ARG1 水平，并探讨血清 EV 衍生的 ARG1 与 T2DM 发生率的关系。
方法
我们在中国进行了一项横断面研究，共纳入 103 名参与者，包括 73 名 T2DM 患者和 30 名非 T2DM 患者。通过超速离心法制备血清 EV。采用酶联免疫吸附试验测定血清 EV 衍生的 ARG1 水平。分析血清 EV 衍生的 ARG1 与临床变量的相关性。采用多变量 logistic 回归分析确定血清 EV 衍生的 ARG1 水平与 T2DM 的关系。采用交互亚组分析评估相关基线对血清 EV 衍生的 ARG1 水平与 T2DM 之间关系的交互作用。
结果
T2DM 患者血清 EV 衍生的 ARG1 水平明显高于非 T2DM 患者（p<0.001）。相关性分析显示，血清 EV 衍生的 ARG1 水平与空腹血糖（FPG）（r=0.316，p=0.001）和糖化血红蛋白（HbA1c）（r=0.322，p=0.001）呈正相关。血清 EV 衍生的 ARG1 水平与 T2DM 显著相关，尤其是在 T2DM 病程超过 10 年的亚组中（OR 1.651，95%CI=1.066-2.557；P 值，0.025），调整混杂因素后。
结论
血清 EV 衍生的 ARG1 浓度升高与 T2DM 密切相关。
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