Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, 605 006, India.
Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, 605 006, India.
Diabetes Metab Syndr. 2020 Nov-Dec;14(6):1751-1755. doi: 10.1016/j.dsx.2020.08.037. Epub 2020 Sep 6.
Carbonyl stress is one of the mechanisms responsible for diabetes and its complications. The study was planned to examine the relationship between carbonyl stress markers and the risk of acute coronary syndrome (ACS) in patients with type 2 diabetes mellitus (T2DM).
Forty T2DM patients with ACS and forty T2DM patients without ACS participated in this cross-sectional pilot study. Routine biochemical investigations, creatine kinase-total (CK-T), and creatine kinase-MB (CK-MB) levels were estimated. Serum carbonyl stress markers were analysed by enzyme-linked immunosorbent assay. Binary logistics regression was done to determine the predictive value of carbonyl stress markers for ACS.
Fasting plasma glucose, serum total methylglyoxal (MG), methylglyoxal derived hydroimidazolones-1 (MG-H1), and N-carboxymethyl-lysine (CML) levels were significantly higher in T2DM patients with ACS than in those without ACS. Serum glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and glyoxalase-1 (GLO1) levels were significantly lower in T2DM patients with ACS than in those without ACS. Fasting plasma glucose level was significantly positively correlated with serum MG (r = 0.441, P < 0.001), CML (r = 0.649, P < 0.001), MG-H1 (r = 0.725, P < 0.001), and negatively correlated with serum GAPDH (r = - 0.268, P = 0.012) and GLO1 (r = - 0.634, P = 0.016). Receiver operating characteristic curve analysis showed that serum GAPDH and GLO1 could predict the risk of ACS in T2DM patients.
These findings revealed that high carbonyl stress due to lower levels of GAPDH and GLO1 may predispose patients with T2DM for more risk of ACS.
羰基应激是导致糖尿病及其并发症的机制之一。本研究旨在探讨 2 型糖尿病（T2DM）患者羰基应激标志物与急性冠状动脉综合征（ACS）风险之间的关系。
本横断面初步研究纳入了 40 例 ACS 合并 T2DM 患者和 40 例非 ACS 合并 T2DM 患者。检测常规生化指标、肌酸激酶总（CK-T）和肌酸激酶-MB（CK-MB）水平。酶联免疫吸附试验分析血清羰基应激标志物。采用二元逻辑回归分析确定羰基应激标志物对 ACS 的预测价值。
ACS 合并 T2DM 患者的空腹血糖、血清总甲基乙二醛（MG）、MG 衍生的羟咪唑酮-1（MG-H1）和 N-羧甲基赖氨酸（CML）水平明显高于非 ACS 合并 T2DM 患者。ACS 合并 T2DM 患者的血清甘油醛-3-磷酸脱氢酶（GAPDH）和糖氧还蛋白-1（GLO1）水平明显低于非 ACS 合并 T2DM 患者。空腹血糖水平与血清 MG（r=0.441，P<0.001）、CML（r=0.649，P<0.001）、MG-H1（r=0.725，P<0.001）呈显著正相关，与血清 GAPDH（r=-0.268，P=0.012）和 GLO1（r=-0.634，P=0.016）呈显著负相关。受试者工作特征曲线分析显示，血清 GAPDH 和 GLO1 可预测 T2DM 患者 ACS 的风险。
这些发现表明，由于 GAPDH 和 GLO1 水平降低导致的高羰基应激可能使 T2DM 患者更容易发生 ACS。
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