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脂质储存滴-2 功能障碍抑制果蝇唾液腺的内复制，并诱导 JNK 通路介导的细胞凋亡。

Dysfunction of lipid storage droplet-2 suppresses endoreplication and induces JNK pathway-mediated apoptotic cell death in Drosophila salivary glands.

机构信息

Department of Functional Chemistry, Kyoto Institute of Technology, Kyoto, 606-8585, Japan.

Faculty of Medical Technology, Hanoi Medical University, Hanoi, 100000, Vietnam.

出版信息

Sci Rep. 2022 Mar 11;12(1):4302. doi: 10.1038/s41598-022-08299-6.

DOI:10.1038/s41598-022-08299-6
PMID:35277579
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8917166/
Abstract

The lipid storage droplet-2 (LSD-2) protein of Drosophila is a homolog of mammalian perilipin 2, which is essential for promoting lipid accumulation and lipid droplet formation. The function of LSD-2 as a regulator of lipolysis has also been demonstrated. However, other LSD-2 functions remain unclear. To investigate the role of LSD-2, we performed tissue-specific depletion in the salivary glands of Drosophila using a combination of the Gal4-upstream activating sequence system and RNA interference. LSD-2 depletion inhibited the entry of salivary gland cells into the endoreplication cycle and delayed this process by enhancing CycE expression, disrupting the development of this organ. The deficiency of LSD-2 expression enhanced reactive oxygen species production in the salivary gland and promoted JNK-dependent apoptosis by suppressing dMyc expression. This phenomenon did not result from lipolysis. Therefore, LSD-2 is vital for endoreplication cell cycle and cell death programs.

摘要

果蝇的脂滴-2（LSD-2）蛋白是哺乳动物 perilipin 2 的同源物，对于促进脂肪积累和脂滴形成至关重要。LSD-2 作为脂肪分解的调节剂的功能也已经得到证实。然而，其他 LSD-2 的功能仍不清楚。为了研究 LSD-2 的作用，我们使用 Gal4-upstream 激活序列系统和 RNA 干扰在果蝇的唾液腺中进行了组织特异性耗竭。LSD-2 耗竭抑制了唾液腺细胞进入内复制周期，并通过增强 CycE 表达、破坏该器官的发育来延迟该过程。LSD-2 表达的缺乏增强了唾液腺中的活性氧产生，并通过抑制 dMyc 表达促进了依赖 JNK 的细胞凋亡。这种现象不是由脂肪分解引起的。因此，LSD-2 对于内复制细胞周期和细胞死亡程序至关重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11bf/8917166/833a3fadd627/41598_2022_8299_Fig9_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11bf/8917166/72238947526b/41598_2022_8299_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11bf/8917166/4be58e0554de/41598_2022_8299_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11bf/8917166/833a3fadd627/41598_2022_8299_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11bf/8917166/f8cc69f71e9b/41598_2022_8299_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11bf/8917166/5a14223412c8/41598_2022_8299_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11bf/8917166/20beb4534c8a/41598_2022_8299_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11bf/8917166/8e52aa64c777/41598_2022_8299_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11bf/8917166/5e9f558fa040/41598_2022_8299_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11bf/8917166/1abde1ec026c/41598_2022_8299_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11bf/8917166/72238947526b/41598_2022_8299_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11bf/8917166/4be58e0554de/41598_2022_8299_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11bf/8917166/833a3fadd627/41598_2022_8299_Fig9_HTML.jpg

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