Department of Functional Chemistry, Kyoto Institute of Technology, Kyoto 606-8585, Japan.
Department of Biology, Can Tho University, Cantho City 900000, Vietnam.
Int J Mol Sci. 2019 Jul 4;20(13):3288. doi: 10.3390/ijms20133288.
Lipin is evolutionarily conserved from yeast to mammals. Although its roles in lipid metabolism in adipocyte tissue, skeletal muscle, and the liver, and as a transcriptional co-activator are known, its functions during development are still under investigation. In this study, we analyzed the role of lipin (dLipin) in development. Specifically, we showed that the tissue-selective knockdown of in the wing pouch led to an atrophied wing. Elevated DNA damage was observed in the wing imaginal disc of -knockdown flies. dLipin dysfunction induced accumulation of cells in S phase and significantly reduced the number of mitotic cells, indicating DNA damage-induced activation of the G2/M checkpoint. Reduced expression of cyclin B, which is critical for the G2 to M transition, was observed in the margin of the wing imaginal disc of -knockdown flies. The knockdown of led to increased apoptotic cell death in the wing imaginal disc. Thus, our results suggest that dLipin is involved in DNA replication during normal cell cycle progression in wing development of .
脂肪酶在从酵母到哺乳动物的进化过程中是保守的。虽然它在脂肪组织、骨骼肌和肝脏中的脂质代谢中以及作为转录共激活因子的作用是已知的，但它在发育过程中的功能仍在研究中。在这项研究中，我们分析了脂肪酶（dLipin）在发育中的作用。具体来说，我们表明，在翅膀囊中的组织选择性敲低导致翅膀萎缩。在 - 敲低果蝇的翅膀 imaginal 盘中观察到 DNA 损伤增加。dLipin 功能障碍诱导 S 期细胞积累，并显著减少有丝分裂细胞的数量，表明 DNA 损伤诱导 G2/M 检查点的激活。在 - 敲低果蝇的翅膀 imaginal 盘边缘观察到细胞周期蛋白 B 的表达降低，这对于 G2 到 M 的转变至关重要。在翅膀 imaginal 盘中观察到 - 敲低导致细胞凋亡增加。因此，我们的结果表明 dLipin 参与了 的翅膀发育过程中正常细胞周期进程中的 DNA 复制。
