Schödel F, Krombach F, Hammer C, Brendel W
Inflammation. 1986 Sep;10(3):271-80. doi: 10.1007/BF00916122.
During organ graft rejection, soluble mediators of inflammation are released into the polymorphs (PMNs) and monocytes recruited from the blood. One functional capacity of polymorphs and monocytes/macrophages is the production of cytotoxic activated oxygen species upon stimulation, which may contribute to the rejection process. Nothing is known about the influence of allograft rejection on this inflammatory cell property. Chemiluminescence (CL) allows measurement of respiratory burst capacity in small cell samples. Zymosan-induced and luminol-amplified CL of diluted whole blood, separated PMNs, and mononuclear cells from peripheral venous blood, as well as of intragraft phagocytes was measured after allogeneic and autologous kidney transplantation in untreated dogs. CL of separated PMNs, mononuclear cells, and intragraft phagocytes was significantly elevated during allograft rejection. In autologous kidneys transplanted to recipients of allografts, CL was also increased in the autologous grafts during rejection of the allogeneic ones, indicating a systemic alteration in phagocyte function.
在器官移植排斥反应过程中，可溶性炎症介质会释放到从血液中募集而来的多形核白细胞（PMNs）和单核细胞中。多形核白细胞以及单核细胞/巨噬细胞的一项功能是在受到刺激时产生具有细胞毒性的活性氧，这可能会促进排斥反应进程。关于同种异体移植排斥反应对这种炎症细胞特性的影响，目前尚不清楚。化学发光（CL）可用于测量小细胞样本中的呼吸爆发能力。在未治疗的犬只进行同种异体和自体肾移植后，测量了稀释全血、分离出的多形核白细胞、外周静脉血中的单核细胞以及移植组织内吞噬细胞的酵母聚糖诱导发光和鲁米诺增强化学发光。在同种异体移植排斥反应期间，分离出的多形核白细胞、单核细胞以及移植组织内吞噬细胞的化学发光显著升高。在将自体肾移植给接受同种异体移植的受体时，在同种异体移植排斥反应期间，自体移植组织中的化学发光也会增加，这表明吞噬细胞功能发生了全身性改变。
