Vadas M A, Nicola N, Lopez A F, Metcalf D, Johnson G, Pereira A
J Immunol. 1984 Jul;133(1):202-7.
Human monocyte-enriched mononuclear cells (MNC) (greater than 95% monocytes) powerfully stimulated the antibody-dependent killing of tumor cells by purified human neutrophils (NE) or eosinophils (EO). The enhancement was observed when the mononuclear cell to granulocyte ratio was as low as 1:100. Media conditioned by MNC also stimulated antibody-dependent killing by NE and EO as well as the uptake and killing of Candida albicans by NE. There was a heterogeneity amongst individuals in the capacity of their MNC to elaborate this factor, and approximately 15% of individuals did not produce detectable factor. Different factors in the MNC supernatant were involved in NE and EO activation. The EO-activating factor (AF) had a m.w. of approximately 27,000 and migrated as a single band on phenyl-Sepharose chromatography. The NE-AF had a m.w. of 22,000 and migrated as two distinct bands on phenyl-Sepharose chromatography. EO-colony-stimulating factor (CSF) co-purified with EO-AF, and granulocyte-macrophage-CSF co-purified with both bands of NE-AF. It is concluded that MNC elaborate CSF-like molecules that powerfully stimulate human granulocyte function.
富含人类单核细胞的单个核细胞（MNC）（单核细胞含量超过95%）能有力地刺激纯化的人类中性粒细胞（NE）或嗜酸性粒细胞（EO）对肿瘤细胞进行抗体依赖性杀伤。当单核细胞与粒细胞的比例低至1:100时，仍可观察到这种增强作用。MNC条件培养基也能刺激NE和EO的抗体依赖性杀伤作用，以及NE对白色念珠菌的摄取和杀伤作用。个体的MNC产生这种因子的能力存在异质性，约15%的个体无法产生可检测到的因子。MNC上清液中的不同因子参与了NE和EO的激活。激活EO的因子（AF）分子量约为27,000，在苯基琼脂糖层析上迁移为单一谱带。激活NE的AF分子量为22,000，在苯基琼脂糖层析上迁移为两条不同的谱带。EO集落刺激因子（CSF）与EO-AF共纯化，粒细胞-巨噬细胞-CSF与NE-AF的两条谱带都共纯化。结论是MNC能产生有力刺激人类粒细胞功能的CSF样分子。
