Department of Medical Physiology, School of Medicine, Abadan University of Medical Sciences, Abadan, Iran.
Department of Biochemistry, School of Medical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Iran Biomed J. 2022 May 1;26(3):219-29. doi: 10.52547/ibj.26.3.219.
This study investigated the antinociceptive effect of cumin and its biosynthesized gold nanoparticles (AuNPs).
Cumin extract (E) and cumin-AuNPs (GN) were prepared and administered intraperitoneally at the concentrations of 200, 500, and 1000 mg/ml to 27 male rats. Ultraviolet-visible spectroscopy and atomic force microscopy were applied for AuNPs synthesis confirmation. The nociceptive behavior was assessed, and IL-6 serum levels were measured.
Cumin-AuNPs showed a peak absorption of 515 nm, and a size of about 40 nm. Three different concentrations of extract had no significant effect on acute and chronic nociceptive behavior. GN + E200 (46.00 ± 10.59) showed a significant acute anti-nociceptive effect compared to the control (98.66 ± 4.91; p = 0.029) and SS300 (98.33 ± 20.30; p = 0.029) groups. Also, GN + E500 (42.00 ± 11.84) significantly reduced acute nociceptive behavior compared to the control (98.66 ± 4.91; p = 0.019), SS300 (98.33 ± 20.30; p = 0.020), and GN + E1000 (91.00 ± 26.00; p = 0.040) groups. IL-6 serum levels reduced significantly in GN + E500 (24.65 ± 10.38; p = 0.002) and SS300 (33.08 ± 1.68; p = 0.039) compared to the controls (46.24 ± 3.02). Chronic nociceptive behavior was significantly lower in the SS300 (255.33 ± 26.30) compared to E200 (477.00 ± 47.29; p = 0.021), E500 (496.25 ± 46.29; p = 0.013), and GN + E500 (437.00 ± 118.03; p = 0.032) groups.
Our findings suggest the potential effects of cumin-AuNPs on formalin-induced nociceptive behavior, which is independent of IL-6serum levels.
本研究旨在探讨孜然及其生物合成的金纳米粒子（AuNPs）的镇痛作用。
制备孜然提取物（E）和孜然-AuNPs（GN），并以 200、500 和 1000mg/ml 的浓度腹腔给药至 27 只雄性大鼠。应用紫外可见光谱和原子力显微镜对 AuNPs 的合成进行确认。评估痛觉行为，并测量血清中白细胞介素-6（IL-6）的水平。
孜然-AuNPs 的最大吸收峰位于 515nm，粒径约为 40nm。三种不同浓度的提取物对急性和慢性痛觉行为均无显著影响。GN+E200（46.00±10.59）与对照组（98.66±4.91；p=0.029）和 SS300（98.33±20.30；p=0.029）相比，表现出显著的急性抗伤害作用。此外，GN+E500（42.00±11.84）与对照组（98.66±4.91；p=0.019）、SS300（98.33±20.30；p=0.020）和 GN+E1000（91.00±26.00；p=0.040）相比，显著降低了急性痛觉行为。GN+E500（24.65±10.38；p=0.002）和 SS300（33.08±1.68；p=0.039）组的血清 IL-6 水平显著低于对照组（46.24±3.02）。与 E200（477.00±47.29；p=0.021）、E500（496.25±46.29；p=0.013）和 GN+E500（437.00±118.03；p=0.032）组相比，SS300 组的慢性痛觉行为显著降低。
我们的研究结果表明，孜然-AuNPs 可能通过影响白细胞介素-6（IL-6）血清水平以外的机制发挥对福尔马林诱导的痛觉行为的潜在作用。
