Er-Rouassi Hafsa, Benichou Luc, Lyoussi Badiaa, Vidal Catherine
Centre Borelli, CNRS UMR-9010, Université de Paris, Paris, France.
Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health, and Quality of Life (SNAMOPEQ), Department of Biology, Faculty of Sciences Dhar Mehraz, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
Front Neurol. 2022 Feb 23;13:827218. doi: 10.3389/fneur.2022.827218. eCollection 2022.
Facial nerve damage can lead to partial or total facial nerve palsy. Photobiomodulation has been reported to improve and accelerate functional recovery following peripheral nerve lesion, depending on the type of lesion and the light exposure parameters used. The aim of this study was to investigate the effects of infrared exposure on functional and axonal regeneration after section-suture of the distal branches of the facial nerve: the buccal and marginal mandibular branches and the distal pes. The animals underwent surgery and were irradiated with infrared light at 850 nm twice daily from day 1 to day 16. The recovery of facial function was then studied at both the behavioral and morphological levels. Behavioral analyses were performed by videoscoring with a high-speed camera and using various devices to assess the recovery of whisker movement on the lesioned side from day 1 to day 30. We also assessed nasal deviation toward the intact side and the ability to close the ipsilateral eyelid completely from day 1 to day 38 and from day 1 to day 50, respectively. For morphological analyses, we assessed the re-establishment of facial motoneuron labeling with Fluorogold®, an immunofluorescent retrograde marker of axonal transport injected into the vibrissae, on D10, D14 and D30. We found that whisker movements recovery was significantly faster in treated than in control mice. A complete disappearance of nasal deviation was observed at 2 weeks in infrared-treated lesioned mice and at 5 weeks in controls. Complete eyelid closure was observed 3 weeks after surgery in treated animals and 6 weeks after surgery in controls. Finally, normal fluorogold labeling of the facial nuclei complex was restored 30 days after surgery in the treated animals, but no such restoration was ever observed in control animals. In conclusion, our data show that IR treatment at a distal site has a significant positive effect on facial nerve recovery. These findings pave the way for the clinical use of infrared photobiomodulation in patients with nerve lesions.
面神经损伤可导致部分或完全性面神经麻痹。据报道，光生物调节可改善并加速周围神经损伤后的功能恢复，这取决于损伤类型和所使用的光照参数。本研究的目的是探讨红外线照射对面神经远端分支（颊支、下颌缘支和远端束）切断缝合后功能和轴突再生的影响。动物接受手术，并从第1天至第16天每天接受两次850 nm红外线照射。然后在行为和形态学水平上研究面部功能的恢复情况。通过高速摄像机进行视频评分，并使用各种设备从第1天至第30天评估损伤侧触须运动的恢复情况，以此进行行为分析。我们还分别从第1天至第38天和第1天至第50天评估了向完整侧的鼻偏斜以及完全闭合同侧眼睑的能力。对于形态学分析，我们在第10天、第14天和第30天评估了用荧光金（一种注入触须的轴突运输免疫荧光逆行标记物）对面部运动神经元标记的重新建立情况。我们发现，接受治疗的小鼠触须运动恢复明显快于对照小鼠。在红外线治疗的损伤小鼠中，2周时观察到鼻偏斜完全消失，而在对照小鼠中则在5周时消失。接受治疗的动物在术后3周观察到眼睑完全闭合，而对照动物在术后6周观察到。最后，接受治疗的动物在术后30天面部核复合体的荧光金标记恢复正常，但对照动物中从未观察到这种恢复。总之，我们的数据表明，远端部位的红外线治疗对面神经恢复有显著的积极影响。这些发现为红外线光生物调节在神经损伤患者中的临床应用铺平了道路。
