Buchaim Daniela Vieira, Andreo Jesus Carlos, Ferreira Junior Rui Seabra, Barraviera Benedito, Rodrigues Antonio de Castro, Macedo Mariana de Cássia, Rosa Junior Geraldo Marco, Shinohara Andre Luis, Santos German Iris Jasmin, Pomini Karina Torres, Buchaim Rogerio Leone
1 Human Morphophysiology (Anatomy), University of Marília (UNIMAR) , Marília, Brazil .
2 Department of Biological Sciences (Anatomy), Bauru School of Dentistry, University of São Paulo , Bauru, Brazil .
Photomed Laser Surg. 2017 Aug;35(8):442-449. doi: 10.1089/pho.2016.4204. Epub 2017 May 26.
Evaluate the efficacy of low-level laser therapy (LLLT) on qualitative, quantitative, and functional aspects in the facial nerve regeneration process.
Forty-two male Wistar rats were used, randomly divided into a control group (CG; n = 10), in which the facial nerve without lesion was collected, and four experimental groups: (1) suture experimental group (SEG) and (2) fibrin experimental group (FEG), consisting of 16 animals in which the buccal branch of the facial nerve was sectioned on both sides of the face; an end-to-end epineural suture was performed on the right side, and a fibrin sealant was used on the left side for coaptation of the stumps; and (3) laser suture experimental group (LSEG) and (4) laser fibrin experimental group (LFEG), consisting of 16 animals that underwent the same surgical procedures as SEG and FEG with the addition of laser application at three different points along the surgical site (pulsed laser of 830 nm wavelength, optical output power of 30 mW, power density of 0.2586 W/cm, energy density of 6.2 J/cm, beam area of 0.116 cm, exposure time of 24 sec per point, total energy per session of 2.16 J, and cumulative dose of 34.56 J). The animals were submitted to functional analysis (subjective observation of whisker movement) and the data obtained were compared using Fisher's exact test. Euthanasia was performed at 5 and 10 weeks postoperative. The total number and density of regenerated axons were analyzed using the unpaired t-test (p < 0.05).
Laser therapy resulted in a significant increase in the number and density of regenerated axons. The LSEG and LFEG presented better scores in functional analysis in comparison with the SEG and FEG.
LLLT enhanced axonal regeneration and accelerated functional recovery of the whiskers, and both repair techniques allowed the growth of axons.
评估低强度激光疗法（LLLT）对面神经再生过程中定性、定量及功能方面的疗效。
选用42只雄性Wistar大鼠，随机分为对照组（CG；n = 10），该组收集未受损的面神经，以及四个实验组：（1）缝合实验组（SEG）和（2）纤维蛋白实验组（FEG），每组16只动物，其双侧面部的颊支面神经被切断；右侧进行端对端神经外膜缝合，左侧使用纤维蛋白密封剂对接断端；（3）激光缝合实验组（LSEG）和（4）激光纤维蛋白实验组（LFEG），每组16只动物，进行与SEG和FEG相同的外科手术，并在手术部位的三个不同点增加激光照射（波长830 nm的脉冲激光，光输出功率30 mW，功率密度0.2586 W/cm，能量密度6.2 J/cm，光束面积0.116 cm，每点照射时间24秒，每次照射总能量2.16 J，累积剂量34.56 J）。对动物进行功能分析（主观观察胡须运动），所得数据采用Fisher精确检验进行比较。术后5周和10周实施安乐死。使用不成对t检验分析再生轴突的总数和密度（p < 0.05）。
激光疗法使再生轴突的数量和密度显著增加。与SEG和FEG相比，LSEG和LFEG在功能分析中表现出更好的评分。
低强度激光疗法增强了轴突再生并加速了胡须的功能恢复，两种修复技术均能使轴突生长。
