Citrus Research Institute, Southwest University, Chongqing 400712, China.
Department of Biology, University of Virginia, Charlottesville, VA 22903, USA.
Genes (Basel). 2020 Mar 7;11(3):284. doi: 10.3390/genes11030284.
VQ motif-containing proteins (VQ proteins) are transcriptional regulators that work independently or in combination with other transcription factors (TFs) to control plant growth and development and responses to biotic and abiotic stresses. VQ proteins contain a conserved FxxhVQxhTG amino acid motif that is the main element of its interaction with WRKY TFs. We identified 59 members of the tobacco ( L.) gene family by in silico analysis and examined their differential expression in response to phytohormonal treatments and following exposure to biotic and abiotic stressors. NtVQ proteins clustered into eight groups based upon their amino acid sequence and presence of various conserved domains. Groups II, IV, V, VI, and VIII contained the largest proportion of gene family members differentially expressed in response to one or more phytohormone, and NtVQ proteins with similar domain structures had similar patterns of response to different phytohormones. genes differentially expressed in response to temperature alterations and mechanical wounding were also identified. Over half of the genes were significantly induced in response to infection. This first comprehensive characterization of the genes in tobacco lays the foundation for further studies of the -mediated regulatory network in plant growth, developmental, and stress-related processes.
VQ motif-containing proteins (VQ proteins) 是转录调控因子，可独立或与其他转录因子（TFs）结合，控制植物的生长发育以及对生物和非生物胁迫的响应。VQ proteins 含有一个保守的 FxxhVQxhTG 氨基酸基序，是其与 WRKY TFs 相互作用的主要元件。我们通过计算机分析鉴定了烟草（ L.）基因家族的 59 个成员，并研究了它们在对植物激素处理和生物及非生物胁迫因子的响应中的差异表达。NtVQ proteins 根据其氨基酸序列和各种保守结构域的存在聚类为八个组。组 II、IV、V、VI 和 VIII 包含最多的对一种或多种植物激素有差异表达的 基因家族成员，并且具有相似结构域的 NtVQ proteins 对不同的植物激素表现出相似的响应模式。还鉴定了对温度变化和机械损伤有差异表达的 基因。超过一半的 基因在响应 感染时显著诱导。这是对烟草中 基因的首次全面描述，为进一步研究植物生长、发育和应激相关过程中的 介导的调控网络奠定了基础。
