Cao Yang, Gao Fei, Chen Wei
Department of Clinical Laboratory, The Second Hospital of Tianjin Medical University, Tianjin, China.
Department of Infectious Diseases, The Second Hospital of Tianjin Medical University, Tianjin, China.
Transl Androl Urol. 2022 Feb;11(2):260-267. doi: 10.21037/tau-22-73.
Midstream urine specimen cultures play an important role in assisting clinicians to choose antibiotics to remove urinary tract pathogens. At present, there are many culture methods for obtaining midstream urine specimens. In this article, different urine culture methods were compared to improve the detection rate of pathogenic bacteria in patients with urinary tract infections (UTIs).
Urina sanguinis specimens were collected from 90 outpatients and inpatients who had UTI symptoms, had been clinically diagnosed with UTI between February and March 2021, and had been tested within 2 h using the conventional 1-µL culture method, the modified 100-µL culture method, and the centrifugal urine sediment culture method. After incubation at 35 °C with 5% carbon dioxide (CO) for 48 h, the detection rates of pathogenic bacteria in UTIs were compared among the three methods.
A total of 75 strains of pathogenic bacteria were detected in the 90 specimens. The positive detection rates of pathogenic microorganisms were 23.33%, 73.33%, and 75.56% for the conventional 1-µL culture method, the modified 100-µL culture method, and the centrifugal urine sediment culture method, respectively. Among the patients who used antibiotics before the collection of the urine specimens, the positive detection rates of pathogenic microorganism were 16.00%, 66.00% and 66.00% for the conventional 1-µL culture method, the modified 100-µL culture method, and the centrifugal urine sediment culture method, respectively. In the specimens grown aseptically using the conventional 1-µL culture method, a similar average number of colonies was found using the modified 100-µL culture method and the centrifugal urine sediment culture method. Among the specimens with bacterial growth in the conventional 1-µL culture method, the average colony numbers of the three methods were similar.
The modified 100-µL culture method and the centrifugal urine sediment culture method greatly improved the positive detection rates of pathogenic bacteria in patients with UTIs.
中段尿标本培养在协助临床医生选择抗生素以清除尿路病原体方面发挥着重要作用。目前，获取中段尿标本有多种培养方法。本文对不同的尿液培养方法进行比较，以提高尿路感染（UTIs）患者病原菌的检出率。
收集2021年2月至3月期间90例有UTI症状、临床诊断为UTI且在2小时内采用传统1-µL培养法、改良100-µL培养法和离心尿沉渣培养法进行检测的门诊和住院患者的血尿标本。在35℃、5%二氧化碳（CO）条件下孵育48小时后，比较三种方法对UTIs病原菌的检出率。
90份标本中共检测出75株病原菌。传统1-µL培养法、改良100-µL培养法和离心尿沉渣培养法的致病微生物阳性检出率分别为23.33%、73.33%和75.56%。在采集尿标本前使用过抗生素的患者中，传统1-µL培养法、改良100-µL培养法和离心尿沉渣培养法的致病微生物阳性检出率分别为16.00%、66.00%和66.00%。在采用传统1-µL培养法无菌生长的标本中，改良100-µL培养法和离心尿沉渣培养法发现的平均菌落数相似。在传统1-µL培养法中有细菌生长的标本中，三种方法的平均菌落数相似。
改良100-µL培养法和离心尿沉渣培养法大大提高了UTIs患者病原菌的阳性检出率。
