Paul J I, Schwarzbauer J E, Tamkun J W, Hynes R O
J Biol Chem. 1986 Sep 15;261(26):12258-65.
Multiple fibronectin mRNAs arise by alternative splicing of the primary transcript of a single gene. We describe analyses of the contribution of this alternative splicing to fibronectin subunit heterogeneity in three different cell types using antisera directed against specific segments of fibronectin. beta-galactosidase-fibronectin fusion proteins produced with the lambda gt11 bacterial expression vector were used as immunogens. One region of alternative splicing accounts for differences in subunit size, while a second contributes to differences between the fibronectins present in blood plasma and in fibroblastic cells. We also show, however, that these two regions of alternative splicing do not account for all detectable subunits. We have also used these segment-specific antisera to show that blood platelets contain a spectrum of fibronectin subunits distinct from that found in blood plasma.
多种纤连蛋白mRNA是由单个基因的初级转录本通过可变剪接产生的。我们使用针对纤连蛋白特定片段的抗血清，描述了在三种不同细胞类型中这种可变剪接对纤连蛋白亚基异质性的贡献分析。用λgt11细菌表达载体产生的β-半乳糖苷酶-纤连蛋白融合蛋白用作免疫原。一个可变剪接区域导致亚基大小的差异，而另一个区域则导致血浆和纤维母细胞中存在的纤连蛋白之间的差异。然而，我们也表明，这两个可变剪接区域并不能解释所有可检测到的亚基。我们还使用这些片段特异性抗血清表明，血小板含有一系列与血浆中不同的纤连蛋白亚基。
