Ayalew Tiruneh Tizezew, Ayalew Tiruneh Gebrehiwot, Chekol Abebe Endeshaw, Mengie Ayele Teklie
Department of Chemistry, College of Natural and Computational Science, University of Gondar, Gondar, Ethiopia.
Department of Midwifery, College of Health Science, Debre Tabor University, Debre Tabor, Ethiopia.
Infect Drug Resist. 2022 Mar 4;15:807-819. doi: 10.2147/IDR.S352049. eCollection 2022.
Among many traditionally used medicinal plants, (Agam) is a well-known indigenous plant in Ethiopia. It is used in its raw form to treat different diseases in different parts of the country. Therefore, the aim of this study is to investigate extraction, isolation, and determination of the antibacterial properties of the solvent leaf extract of
In this study, 800 g of powdered leaves of were macerated with 2500 mL of methanol and yielded 58 g (7.25%, w/w) of gummy material. The extract was then further partitioned by using ethyl acetate and chloroform. The extracts were subjected to phytochemical screening test. The antibacterial activity of the three solvent leaf extracts of were evaluated using disc diffusion method. The methanol extract was subjected to column chromatography silica gel (60-200 mesh) by mixing methanol:petroleum ether (4:1). Then fractions were collected and investigated by TLC and finally identified using spectroscopy.
The three extracts (methanol, ethyl acetate, and chloroform) of were presented to antimicrobial activity by disc diffusion method against four bacterial species using gentamycin and ampicillin discs as positive controls and DMSO as a negative control. All extracts had a relatively antibacterial effect with different extent zones of inhibition. However, the methanol extract showed superior antibacterial activity compared with DMSO and ethyl acetate and chloroform extracts. These could due to variation of the phytoconstituents. The most probable structure of the compound isolated was 5-(2',3',4',6'-tetrahydroxy-5'-methoxycyclohexyloxy)-2,3,4 trihydroxypentanoic acid.
Data obtained from this study collectively indicated that the three solvent extracts of have a promising antimicrobial activity which supports the traditional claim of the plant for treatment of infection.
在许多传统药用植物中，（阿加姆）是埃塞俄比亚一种著名的本土植物。它以原始形态在该国不同地区用于治疗各种疾病。因此，本研究的目的是调查[植物名称]溶剂叶提取物的提取、分离及其抗菌特性的测定。
在本研究中，800克[植物名称]的粉末状叶子用2500毫升甲醇浸渍，得到58克（7.25%，w/w）的胶状物质。然后用乙酸乙酯和氯仿对提取物进行进一步分离。对提取物进行植物化学筛选试验。采用纸片扩散法评估[植物名称]三种溶剂叶提取物的抗菌活性。甲醇提取物通过将甲醇：石油醚（4:1）混合，在硅胶柱（60 - 200目）上进行柱色谱分离。然后收集馏分，通过薄层色谱法进行研究，最后用光谱法进行鉴定。
采用纸片扩散法，以庆大霉素和氨苄青霉素纸片作为阳性对照，二甲基亚砜作为阴性对照，对[植物名称]的三种提取物（甲醇、乙酸乙酯和氯仿）针对四种细菌进行抗菌活性测试。所有提取物都有不同程度的抑菌圈，表现出相对的抗菌效果。然而，与二甲基亚砜以及乙酸乙酯和氯仿提取物相比，甲醇提取物显示出更强的抗菌活性。这可能是由于植物成分的差异。分离得到的化合物最可能的结构是5 -（2'，3'，4'，6' - 四羟基 - 5' - 甲氧基环己氧基）- 2，3，4 - 三羟基戊酸。
本研究获得的数据共同表明，[植物名称]的三种溶剂提取物具有良好的抗菌活性，这支持了该植物用于治疗感染的传统说法。
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