不同溶剂提取筋骨草叶片提取物的体外抗氧化和抗菌活性。
In vitro antioxidant and antibacterial activities of Ajuga integrifolia leaf extracts obtained with different solvents.
机构信息
Department of Chemistry, and Energy and Environment Research Center, Dilla University, P. O. Box 419, Dilla, Ethiopia.
Department of Chemistry, Hawassa College of Teacher Education, P. O. Box 115, Hawassa, Ethiopia.
出版信息
BMC Complement Med Ther. 2024 Oct 12;24(1):368. doi: 10.1186/s12906-024-04668-y.
BACKGROUND
Many diseases are increasingly recognized as public health concerns worldwide because of the increasing incidence of multidrug-resistant bacteria. Recently, interest in the use of indigenous medicinal plants to treat infectious illnesses has increased, highlighting the need to find new bioactive phytochemicals. Ajuga integrifolia is a plant commonly utilized in traditional drugs to treat a wide range of diseases, although its effectiveness has not been scientifically validated. The present study aimed to evaluate the total phenolic and flavonoid contents and assess the biological activities of A. integrifolia leaf extracts produced via different solvent systems.
METHODS
Soxhlet extraction was employed to obtain crude extracts from different solvents (methanol, ethanol and water). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric-reducing power assays were used to measure the antioxidant activity, and the antibacterial activity of the extract was evaluated on the basis of its minimum inhibitory concentration (MIC) against two gram-negative bacteria (Escherichia coli (ATCC-25922) and Pseudomonas aeruginosa (ATCC-43495)) and two gram-positive bacteria (Staphylococcus aureus (ATCC-25923) and Streptococcus pyogenes (ATCC-19615)) via the agar disk-diffusion technique.
RESULTS
A significant amount of total phenolic content (TPC) and flavonoid content (TFC) were present in all the extracts. The extracts presented powerful antioxidant activity in all the assays. The disc diffusion and MIC results revealed the ability of the methanol and ethanol extracts of A. integrifolia leaves to inhibit S. aureus growth at a concentration of 3.125 mg/mL. However, the water extracts were ineffective against E. coli and P. aeruginosa.
CONCLUSIONS
These findings indicate that A. integrifolia leaf extracts have reasonable biological activities. These findings underscore the importance of A. integrifolia leaves as a source of health benefits.
背景
由于多药耐药菌的发病率不断增加，许多疾病已日益被视为全球公共卫生关注的问题。最近，人们对利用本土药用植物治疗传染病的兴趣日益浓厚，这凸显了寻找新的生物活性植物化学物质的必要性。筋骨草是一种常用于传统药物的植物，可治疗多种疾病，尽管其疗效尚未得到科学验证。本研究旨在评估筋骨草叶不同溶剂系统提取物的总酚和类黄酮含量，并评估其生物活性。
方法
采用索氏提取法从不同溶剂（甲醇、乙醇和水）中提取粗提物。采用 2,2-二苯基-1-苦基肼（DPPH）和铁还原能力测定法来测定抗氧化活性，根据其对两种革兰氏阴性菌（大肠杆菌（ATCC-25922）和铜绿假单胞菌（ATCC-43495））和两种革兰氏阳性菌（金黄色葡萄球菌（ATCC-25923）和化脓性链球菌（ATCC-19615））的最低抑菌浓度（MIC），通过琼脂平板扩散法来评估提取物的抗菌活性。
结果
所有提取物均含有大量的总酚含量（TPC）和类黄酮含量（TFC）。所有提取物在所有测定中均表现出强大的抗氧化活性。通过圆盘扩散法和 MIC 结果表明，筋骨草叶甲醇和乙醇提取物在 3.125mg/mL 浓度下能够抑制金黄色葡萄球菌的生长。然而，水提取物对大肠杆菌和铜绿假单胞菌无效。
结论
这些发现表明筋骨草叶提取物具有合理的生物活性。这些发现强调了筋骨草叶作为健康益处来源的重要性。
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