评估美洲蒲桃树枝上的安息香树叶的植物化学成分和抗菌性能。

Assessment of the phytochemical composition and antimicrobial properties of Tapinanthus bangwensis leaves hosted by the branches of Persea americana.

机构信息

Phytochemistry Department, Centre for Plant Medicine Research, Mampong-Akuapem, Ghana.

Department of Microbiology, Centre for Plant Medicine Research, Mampong-Akuapem, Ghana.

出版信息

BMC Complement Med Ther. 2023 Feb 3;23(1):34. doi: 10.1186/s12906-023-03860-w.

DOI:10.1186/s12906-023-03860-w

PMID:36737748

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9896793/

Abstract

BACKGROUND

Medicinal plants represent a valuable source for new effective and safe antimicrobial drugs making them an alternative therapy. Existing antimicrobial agents are costly and mostly associated with possible side effects. The aim of the present study therefore, was to assess the antimicrobial property and phytochemical composition of hydroethanolic extract of Tapinanthus bangwensis leaves and its fractions.

METHOD

T. bangwensis leaves (harvested from its host plant, Persea americana) was extracted by cold maceration with 70% ethanol and further fractionated with different organic solvents using the solvent partitioning method to obtain the crude extract, petroleum ether, chloroform, ethyl acetate and the resulting aqueous fractions. The phytochemical constituents of the extracts were screened and quantified. Also, the TLC of the extracts were analyzed to serve as a fingerprint. Using the agar diffusion and broth dilution methods, the antimicrobial properties of the extracts were assessed.

RESULTS

The study showed that the hydroethanolic (70%) crude extract of T. bangwensis leaves and its fractions contain phenolic compounds, flavonoids, saponins, phytosterols and reducing sugars. The phytoconstituents were well extracted into the ethyl acetate fraction than the other fractions evidenced in the high levels (p < 0.0001) of saponins (66.47 ± 1.72% w/w), phenolic compounds (77.75 ± 1.06 mg/100 mg GAE) and flavonoids (44.34 ± 0.06 mg/100 mg QE) contents. From the antimicrobial studies, all the microorganisms tested exhibited varying degrees of susceptibility to the extracts with MIC values between 0.78 to 12.5 mg/mL. The crude extract of T. bangwensis leaves, its ethyl acetate and chloroform fractions also exhibited lethal antimicrobial activity with MLC between 6.25 to 50 mg/mL.

CONCLUSION

The crude extract of T. bangwensis leaves and its fractions demonstrated antimicrobial properties against Escherichia coli, Staphylococcus aureus, Staphylococcus saprophyticus and Candida albicans, thereby representing a potential source of natural antimicrobial agent. Further study is required to identify and isolate antimicrobial compounds from the plant for the development of the natural bioactive antimicrobial agents.

摘要

背景

药用植物是新的有效和安全的抗菌药物的宝贵来源，它们是一种替代疗法。现有的抗菌药物成本高昂，且大多与可能的副作用有关。因此，本研究旨在评估 Tapinanthus bangwensis 叶及其提取物的水醇提取物的抗菌特性和植物化学成分。

方法

采用冷浸法用 70%乙醇从宿主植物鳄梨（Persea americana）中提取 Tapinanthus bangwensis 叶，然后采用溶剂分配法用不同有机溶剂进一步分离，得到粗提物、石油醚、氯仿、乙酸乙酯和水提物。对提取物中的植物化学成分进行筛选和定量。此外，还分析了提取物的 TLC，作为指纹图谱。采用琼脂扩散和肉汤稀释法评估提取物的抗菌性能。

结果

研究表明，Tapinanthus bangwensis 叶的水醇（70%）粗提取物及其各部位均含有酚类化合物、类黄酮、皂苷、植物甾醇和还原糖。植物成分很好地提取到乙酸乙酯部位，其皂苷（66.47±1.72%w/w）、酚类化合物（77.75±1.06 mg/100 mg GAE）和类黄酮（44.34±0.06 mg/100 mg QE）含量均明显高于其他部位（p<0.0001）。从抗菌研究结果来看，所有测试的微生物对提取物的敏感性均不同，MIC 值在 0.78 至 12.5 mg/mL 之间。Tapinanthus bangwensis 叶的粗提取物及其乙酸乙酯和氯仿部位也表现出致死性抗菌活性，其 MLC 在 6.25 至 50 mg/mL 之间。