Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China.
University of Science and Technology of China, Hefei, China.
PeerJ. 2022 Mar 7;10:e13078. doi: 10.7717/peerj.13078. eCollection 2022.
Hydrogen sulphide (HS), a crucial gas signal molecule, has been reported to be involved in various processes related to development and adversity responses in plants. However, the effects and regulatory mechanism of HS in controlling head blight (FHB) in wheat have not been clarified. In this study, we first reported that HS released by low concentrations of sodium hydrosulphide (NaHS) could significantly alleviate the FHB symptoms caused by () in wheat. We also used coleoptile inoculation to investigate the related physiological and molecular mechanism. The results revealed that FHB resistance was strongly enhanced by the HS released by NaHS, and 0.3 mM was confirmed as the optimal concentration. HS treatment dramatically reduced the levels of hydrogen peroxide (HO) and malondialdehyde (MDA) while enhancing the activities of antioxidant enzymes. Meanwhile, the relative expressions levels of defence-related genes, including , , , and , were all dramatically upregulated. Our results also showed that HS was toxic to by inhibiting mycelial growth and spore germination. Taken together, the findings demonstrated the potential value of HS in mitigating the adverse effects induced by and advanced the current knowledge regarding the molecular mechanisms in wheat.
硫化氢（HS）作为一种重要的气体信号分子，已被报道参与植物发育和逆境响应的多个过程。然而，HS 控制小麦赤霉病（FHB）的作用及其调控机制尚不清楚。本研究首次报道低浓度的硫氢化钠（NaHS）释放的 HS 可显著减轻小麦中 引起的 FHB 症状。我们还使用胚芽鞘接种法研究了相关的生理和分子机制。结果表明，HS 释放强烈增强了小麦对 FHB 的抗性，并且 0.3mM 被确认为最佳浓度。HS 处理显著降低了过氧化氢（HO）和丙二醛（MDA）的水平，同时增强了抗氧化酶的活性。同时，防御相关基因的相对表达水平，包括 、 、 、和 ，均显著上调。我们的结果还表明，HS 通过抑制菌丝生长和孢子萌发对 具有毒性。总之，这些发现表明 HS 在减轻 诱导的不利影响方面具有潜在价值，并推进了小麦中分子机制的现有知识。
