Fanelli Diana, Tesi Matteo, Monaco Davide, Diaz-Jimenez Maria, Camillo Francesco, Rota Alessandra, Panzani Duccio
Veterinary Sciences Department, Pisa University, Pisa, Italy.
Veterinary Sciences Department, Pisa University, Pisa, Italy.
J Equine Vet Sci. 2022 May;112:103932. doi: 10.1016/j.jevs.2022.103932. Epub 2022 Mar 10.
In the literature, the very low pregnancy rates after artificial insemination (AI) with frozen semen in donkeys were improved in one study after re-extension of the frozen-thawed semen in autologous seminal plasma. The aims of our study were (1) to describe in vitro post-thaw parameters of donkey jackass semen after re-extension in seminal plasma (SP) or in INRA96 and (2) to compare pregnancy rates in jennies bred with frozen-thawed semen using two different AI protocols. Semen collected from two Amiata donkey stallions, known to be fertile, was frozen in INRA96 supplemented with 2% egg yolk, and 2.2% glycerol. From the same jacks, seminal plasma was filtered, and stored at -20°C. According to the in vitro analysis, the post-thaw re-extension in SP some kinematic parameters decreased more over time. Two different AI protocols were tested: inseminating deeply into the uterine horn (DHAI) with or without thawed semen re-extension in SP. Higher pregnancy rates were obtained with re-extension in SP than when AIs were performed without SP. Further studies are needed to confirm the advantage of re-extension in autologous SP of donkey frozen-thawed semen before AI in jennies.
在文献中，一项研究表明，在驴中使用冷冻精液进行人工授精（AI）后极低的妊娠率，在将冻融精液在自体精浆中重新稀释后有所提高。我们研究的目的是：（1）描述驴种公马精液在精浆（SP）或INRA96中重新稀释后的体外解冻后参数；（2）比较使用两种不同AI方案，用冻融精液对母驴进行配种后的妊娠率。从两匹已知具有生育能力的阿米亚塔驴种公马采集的精液，在添加2%蛋黄和2.2%甘油的INRA96中冷冻。从同一公马采集精浆，过滤后储存在-20°C。根据体外分析，在SP中解冻后重新稀释，一些运动学参数随时间下降得更多。测试了两种不同的AI方案：将解冻精液在SP中重新稀释或不重新稀释，然后深部子宫角授精（DHAI）。与不使用SP进行AI相比，在SP中重新稀释获得了更高的妊娠率。在母驴AI前，还需要进一步研究来证实驴冻融精液在自体SP中重新稀释的优势。
