Seyedasgari Fahimeh, Asadi Behnam, Kim Ellen
Camel Advanced Reproductive Center, Zabeel Office, Government of Dubai, Dubai 5928, United Arab Emirates.
Anim Biosci. 2023 Dec;36(12):1821-1830. doi: 10.5713/ab.23.0136. Epub 2023 Aug 23.
This study investigated the effect of adding seminal plasma to frozen-thawed semen on the quality of sperm and pregnancy following insemination in dromedary camels.
In experiment 1, the frozen-thawed semen from 9 collections (3 bulls) was further diluted with either the base extender or homologous seminal plasma (HSP). In the second experiment, a pooled sample of frozen-thawed semen was diluted with either seminal plasma from another three bulls. Live percentage, total and progressive motility, functional and acrosome integrity, and sperm kinematics were evaluated at 15, 60, and 120 minutes post-thawing and compared to the non-treated control. In experiment 3, frozen semen was used to inseminate camels in the following experimental groups: 1-Single insemination with double dose undiluted frozen semen (n = 9); 2-Re-insemination in 6 hours with undiluted semen (n = 13); 3-Single insemination with HSP treated sperm (n = 14).
Frozen-thawed sperm diluted in HSP or the non-homologous seminal plasma from Bull C indicated an improvement in all parameters after 1 hour post-thawing incubation (p<0.05). The proportion of total and progressively motile sperm did not drop significantly at 60 minutes post-thawing when diluted with the seminal plasma of Bull C (p>0.05). Double insemination with nontreated sperm and single insemination with HSP-treated sperm resulted in similar pregnancy rates (15.3% vs 21.4%, p>0.05). None of the camels conceived with double-dose single insemination of nontreated sperm.
Seminal plasma improves sperm longevity and motility after thawing in dromedary camel with a significant between-bull variation in effect. Low post-thaw sperm longevity might be the cause behind the low pregnancy rates in frozen semen insemination of dromedary camels.
本研究调查了在单峰骆驼中，向冻融精液中添加精浆对精子质量和授精后妊娠情况的影响。
在实验1中，将来自9次采集（3头公牛）的冻融精液用基础稀释液或同源精浆（HSP）进一步稀释。在第二个实验中，将冻融精液的混合样本用另外三头公牛的精浆进行稀释。在解冻后15、60和120分钟评估活率、总活力和前向运动活力、功能完整性和顶体完整性以及精子运动学，并与未处理的对照组进行比较。在实验3中，使用冷冻精液对以下实验组的骆驼进行授精：1-单次授精，使用双倍剂量未稀释的冷冻精液（n = 9）；2-6小时后用未稀释精液再次授精（n = 13）；3-单次授精，使用经HSP处理的精子（n = 14）。
用HSP或公牛C的非同源精浆稀释的冻融精子在解冻后孵育1小时后所有参数均有改善（p<0.05）。用公牛C的精浆稀释时，解冻后60分钟时总活动精子和前向运动精子的比例没有显著下降（p>0.05）。未处理精子的双倍授精和经HSP处理精子的单次授精导致相似的妊娠率（15.3%对21.4%，p>0.05）。没有骆驼通过未处理精子的双倍剂量单次授精受孕。
精浆可提高单峰骆驼解冻后精子的寿命和活力，且公牛之间的效应存在显著差异。解冻后精子寿命低可能是单峰骆驼冷冻精液授精妊娠率低的原因。
