da Costa Ohanna Maria Menezes M, Firmino Priscilla C O S, Strapasson Guilherme B, de la Torre Lucimara G, Malfatti-Gasperini Antonio A, Júnior Severino Alves
Department of Fundamental Chemistry (DQF), Federal University of Pernambuco.
Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM).
J Oleo Sci. 2022 Apr 1;71(4):515-522. doi: 10.5650/jos.ess21309. Epub 2022 Mar 14.
Anionic liposomes (AL) are very attractive for nanomedicine and some formulations have already been launched for clinical development. Despite the excellent potential, their application presents two major challenges: laborious production methods and rapid degradation and elimination from blood by the immune system. In this work, we optimized the production of AL and its stealth form (SAL) using a onestep microfluidic process. We obtained unilamellar and near-monodisperse (< 10%) AL composed by the commercial composition (DMPC:DMPG) with mean size small as 53.7 nm, which is optimized for application in drug delivery. We also obtained SAL with similar characteristics using the microfluidic technique, overcoming the limitation of conventional methods where SAL presents high polydispersity (> 30%). This study demonstrates the great potential of the microfluidic technique for one-step production of stealth anionic nanoliposomes with controlled sizes and reproducible characteristics.
阴离子脂质体（AL）在纳米医学领域极具吸引力，一些制剂已进入临床开发阶段。尽管具有巨大潜力，但其应用面临两大挑战：生产方法繁琐，以及在血液中会被免疫系统迅速降解和清除。在这项工作中，我们使用一步微流控工艺优化了AL及其隐形形式（SAL）的生产。我们获得了由商业配方（DMPC:DMPG）组成的单层且近乎单分散（<10%）的AL，其平均尺寸小至53.7nm，非常适合用于药物递送。我们还使用微流控技术获得了具有类似特性的SAL，克服了传统方法中SAL具有高多分散性（>30%）的局限性。这项研究证明了微流控技术在一步生产具有可控尺寸和可重复特性的隐形阴离子纳米脂质体方面的巨大潜力。
