槲皮素和姜黄素在二甲基亚砜水溶液及乙醇水溶液中与人血清白蛋白的结合
Binding of quercetin and curcumin to human serum albumin in aqueous dimethyl sulfoxide and in aqueous ethanol.
作者信息
Usacheva Tatyana, Gamov George, Bychkova Anna, Anufrikov Yuriy, Shasherina Anna, Alister Diana, Kuranova Natalya, Sharnin Valentin
机构信息
Ivanovo State University of Chemistry and Technology, Ivanovo, Russia.
N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.
出版信息
J Therm Anal Calorim. 2022;147(9):5511-5518. doi: 10.1007/s10973-022-11216-8. Epub 2022 Mar 5.
UNLABELLED
The paper reports the spectrofluorimetric and calorimetric study of binding of two hydrophobic biologically active molecules with antioxidant ability, flavonoids quercetin, and curcumin, to human serum albumin (HSA) in water, aqueous DMSO (0.05 and 0.1 mol. fraction of DMSO), and aqueous ethanol (0.05 mol. fraction of EtOH). Both flavonoids induce the quenching of HSA fluorescence. The stability constants of associates, as well as the changes in enthalpy of the reaction between quercetin and protein, were evaluated. The influence of solvent composition and additions of hydroxypropyl-β-cyclodextrin as a solubilizer of hydrophobic molecules, on the association processes is discussed.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s10973-022-11216-8.
未标记
本文报道了两种具有抗氧化能力的疏水性生物活性分子——黄酮类化合物槲皮素和姜黄素，在水、含二甲基亚砜（DMSO）的水溶液（DMSO的摩尔分数为0.05和0.1）以及含乙醇的水溶液（乙醇的摩尔分数为0.05）中与人类血清白蛋白（HSA）结合的荧光光谱和量热研究。两种黄酮类化合物均能诱导HSA荧光猝灭。评估了缔合物的稳定常数以及槲皮素与蛋白质反应的焓变。讨论了溶剂组成以及添加羟丙基-β-环糊精作为疏水分子增溶剂对缔合过程的影响。
补充信息
在线版本包含可在10.1007/s10973-022-11216-8获取的补充材料。
Zotero 插件