United States Department of Agriculture - Agricultural Research Service - Western Regional Research Center, 800 Buchanan Street, 94710, Albany, CA, USA.
Sci Rep. 2019 Feb 14;9(1):2016. doi: 10.1038/s41598-019-38769-3.
Two natural compounds (quercetin and curcumin) were tested as sensitizing or protecting agents for Navel Orangeworm (NOW) larvae under x-ray sterilization, with the aim to reduce required doses and thus facilitate the substitution of x-ray for radioisotopes. The compounds were added to NOW diet at concentrations between 0 and 1.0 mmol kg and subsequent reared male larvae were subjected to x-ray irradiation (90 keV, 9 mA) to doses up to 15 Gy. Upon emergence as adults, surviving male NOW were paired with colony virgin females and placed in isolation for observation of deformity, mortality, and fertility. Treatments included rearing larvae on infused diet before irradiation, after irradiation, and both. Results were tabulated as percentage of insects that were dead/deformed, infertile, or fertile and subjected to chi-squared analysis. While insect populations subjected to quercetin treatments were not found to be significantly different from control at any x-ray dose, all curcumin treatments yielded significant differences at an absorbed dose of 10 Gy, both in terms of decreased mortality and fertility. While none of the treatments resulted in acceptable mortality/deformity rates, the observed effects strongly support the need for continued testing of natural compounds for their efficacy to reduce required dose levels for sterilization.
两种天然化合物（槲皮素和姜黄素）被测试为射线灭菌下 Navel Orangeworm（NOW）幼虫的敏化或保护剂，目的是减少所需剂量，从而促进射线取代放射性同位素。这些化合物以 0 到 1.0 mmol/kg 的浓度添加到 NOW 饮食中，随后饲养的雄性幼虫接受高达 15 Gy 的 X 射线照射（90keV，9mA）。成虫出现后，幸存的雄性 NOW 与 colony 处女雌性配对，并将其隔离观察畸形、死亡率和生育能力。处理包括在照射前、照射后和两者都进行幼虫在注入饮食中的饲养。结果以死亡/畸形、不育或可育昆虫的百分比表示，并进行卡方分析。虽然在任何 X 射线剂量下，接受槲皮素处理的昆虫种群与对照相比没有发现显著差异，但在吸收剂量为 10 Gy 时，所有姜黄素处理都产生了显著差异，死亡率和生育力都降低了。虽然没有一种处理方法导致可接受的死亡率/畸形率，但观察到的效果强烈支持继续测试天然化合物的功效，以降低灭菌所需的剂量水平。
