Soltani Navid, Rabbany Esfahany Elham, Druzhinin Sergey I, Schulte Gregor, Müller Julian, Butz Benjamin, Schönherr Holger, Agio Mario, Markešević Nemanja
Laboratory of Nano-Optics, University of Siegen, Siegen 57072, Germany.
Research Center of Micro- and Nanochemistry and (Bio)Technology (Cμ), Siegen 57076, Germany.
Biomed Opt Express. 2022 Jan 3;13(2):539-548. doi: 10.1364/BOE.445402. eCollection 2022 Feb 1.
We investigate a model bioassay in a liquid environment using a -scanning planar Yagi-Uda antenna, focusing on the fluorescence collection enhancement of ATTO-647N dye conjugated to DNA (deoxyribonucleic acid) molecules. The antenna changes the excitation and the decay rates and, more importantly, the emission pattern of ATTO-647N, resulting in a narrow emission angle (41°) and improved collection efficiency. We efficiently detect immobilized fluorescently-labeled DNA molecules, originating from solutions with DNA concentrations down to 1 nM. In practice, this corresponds to an ensemble of fewer than 10 ATTO-647N labeled DNA molecules in the focal area. Even though we use only one type of biomolecule and one immobilization technique to establish the procedure, our method is versatile and applicable to any immobilized, dye-labeled biomolecule in a transparent solid, air, or liquid environment.
我们使用扫描平面八木-宇田天线研究了液体环境中的一种模型生物测定法，重点关注与DNA（脱氧核糖核酸）分子共轭的ATTO-647N染料的荧光收集增强。该天线改变了ATTO-647N的激发和衰减速率，更重要的是改变了其发射模式，从而产生了狭窄的发射角（41°）并提高了收集效率。我们能够有效地检测固定化的荧光标记DNA分子，这些分子来自DNA浓度低至1 nM的溶液。实际上，这对应于焦点区域中少于10个ATTO-647N标记的DNA分子的集合。尽管我们仅使用一种生物分子和一种固定化技术来建立该程序，但我们的方法具有通用性，适用于透明固体、空气或液体环境中任何固定化的、染料标记的生物分子。
