Manalili Dexter, Berardi Massimiliano, Aardema Hilde, Asimaki Konstantina, Sarmiento Raymund, Imran Akca B
LaserLab, Vrije Universiteit Amsterdam, De Boelelaan, Amsterdam 1081 HV, The Netherlands.
Physics Department, University of San Carlos, Talamban, Cebu City 6000, Philippines.
Biomed Opt Express. 2022 Jan 18;13(2):824-837. doi: 10.1364/BOE.447147. eCollection 2022 Feb 1.
The parallel-plate compression test is one of the simplest ways to measure the mechanical properties of a material. In this test, the Young's modulus ( ) and the Poisson's ratio ( ) of the material are determined directly without applying any additional modelling and parameter fitting in the post-processing. This is, however, limited when dealing soft biological materials due to their inherent properties such as being inhomogeneous, microscopic, and overly compliant. By combining an interferometry-assisted parallel-plate compression system and a confocal microscope, we were able to overcome these limitations and measure the (315 52 Pa) and (0.210 0.043) of fixated and permeabilized bovine oocytes.
平行板压缩试验是测量材料力学性能的最简单方法之一。在该试验中，材料的杨氏模量（ ）和泊松比（ ）可直接测定，后处理过程中无需进行任何额外的建模和参数拟合。然而，在处理柔软的生物材料时，由于其固有的不均匀、微观和过度柔顺等特性，这种方法存在局限性。通过将干涉测量辅助平行板压缩系统与共聚焦显微镜相结合，我们能够克服这些局限性，并测量固定和透化处理后的牛卵母细胞的杨氏模量（315±52 Pa）和泊松比（0.210±0.043）。
