LaserLab, Department of Physics and Astronomy, VU University, De Boelelaan 1081, Amsterdam, The Netherlands.
Optics11, De Boelelaan 1081, Amsterdam, The Netherlands.
Commun Biol. 2021 May 21;4(1):610. doi: 10.1038/s42003-021-02121-1.
Micropipette aspiration (MPA) is an essential tool in mechanobiology; however, its potential is far from fully exploited. The traditional MPA technique has limited temporal and spatial resolution and requires extensive post processing to obtain the mechanical fingerprints of samples. Here, we develop a MPA system that measures pressure and displacement in real time with sub-nanometer resolution thanks to an interferometric readout. This highly sensitive MPA system enables studying the nanoscale behavior of soft biomaterials under tension and their frequency-dependent viscoelastic response.
微量吸管吸移（MPA）是机械生物学中的基本工具；然而，它的潜力还远未得到充分开发。传统的 MPA 技术的时间和空间分辨率有限，并且需要进行广泛的后处理才能获得样品的力学指纹。在这里，我们开发了一种 MPA 系统，该系统借助干涉仪读数能够实时以亚纳米分辨率测量压力和位移。这种高灵敏度的 MPA 系统能够研究拉伸下软生物材料的纳米级行为及其频率相关的粘弹性响应。
