Yeh K Y, Du F W, Holt P R
J Nutr. 1986 Jul;116(7):1334-42. doi: 10.1093/jn/116.7.1334.
The effects of sucrose and corticosterone on the expression of intestinal sucrase activity in preweanling rat pups were studied using an artificial rearing (AR) technique. When AR rat pups were isocalorically fed diets containing lactose or sucrose, or a carbohydrate-free diet from d 12-16, jejunal and ileal sucrase and maltase activities were induced to similar levels in all AR rats, whereas ileal lactase activity was precociously decreased. In separate experiments, enzyme activities were measured in ileal isografts subcutaneously implanted in littermates at birth. In AR rats fed the lactose diet, these isografts showed sucrase and maltase activities comparable with those of host ileum and also to isografts from AR rats fed the sucrose diet. In contrast, lactase activity was significantly higher in isografts than host ileum in all AR rats. Serum corticosterone levels were significantly elevated in AR rats for 24 h after intragastric cannular implantation. Precocious expression of ileal sucrase activity occurred in corticosterone treated, but not in untreated, adrenalectomized AR rats. In conclusion, dietary sucrose has no specific role in enhancing intestinal sucrase activity, and endogenous corticosterone is responsible for the induction of sucrase activity in AR rats.
采用人工饲养（AR）技术，研究了蔗糖和皮质酮对断奶前大鼠幼崽肠道蔗糖酶活性表达的影响。当从第12至16天给AR大鼠幼崽等热量地喂食含乳糖或蔗糖的日粮，或无碳水化合物日粮时，所有AR大鼠空肠和回肠的蔗糖酶及麦芽糖酶活性均被诱导至相似水平，而回肠乳糖酶活性则过早降低。在单独的实验中，对出生时皮下植入同窝幼崽体内的回肠同种移植物的酶活性进行了测定。在喂食乳糖日粮的AR大鼠中，这些同种移植物的蔗糖酶和麦芽糖酶活性与宿主回肠相当，也与喂食蔗糖日粮的AR大鼠的同种移植物相当。相反，在所有AR大鼠中，同种移植物中的乳糖酶活性显著高于宿主回肠。胃内插管植入后24小时，AR大鼠的血清皮质酮水平显著升高。在经皮质酮处理的去肾上腺AR大鼠中出现了回肠蔗糖酶活性的过早表达，而在未处理的去肾上腺AR大鼠中则未出现。总之，日粮中的蔗糖在增强肠道蔗糖酶活性方面没有特定作用，内源性皮质酮是AR大鼠中蔗糖酶活性诱导的原因。
