作为甲状腺癌独立预后指标的高表达。 （注：原文中“as an independent prognostic Indicator in thyroid carcinoma”前面缺少具体的表达内容，比如某个基因、蛋白等的高表达，这里翻译是按照补充完整后的意思来呈现的，原英文表述不太完整规范）
High expression of as an independent prognostic Indicator in thyroid carcinoma.
作者信息
Luo Xue, Chen Xinghong, Chen Song, Gao Qingjun, Yang Huifang, Zhao Daiwei
机构信息
Clinical Medical College, Guizhou Medical University, Guiyang, China.
Department of Thyroid Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang, China.
出版信息
Gland Surg. 2022 Feb;11(2):412-425. doi: 10.21037/gs-22-37.
BACKGROUND
Seizure-related 6 homolog (mouse)-like 2 () is a type 1 transmembrane protein that is primarily expressed in the brain . In recent reports, has been found to be overexpressed in some cancers and can drive the progression of tumors. However, its function and mechanism in thyroid cancer remain unclear.
METHODS
In this article ,we searched for the expressions in Pan-cancer on TCGA (The Cancer Genome Atlas) and evaluated these data using the TIMER2 method. Then, the immunohistochemical score (IHC score) of in cancer tissue was collected in human protein mapping (HPA) data. And we used CIBERSORT to assess the association between the levels of expression and the number of various immune cells in papillary thyroid carcinoma (PTC) tissue. Finally, Gene Expression Omnibus (GEO) analyses, real-time quantitative polymerase chain reaction (qRT-PCR) of tissues, and immunohistochemical staining were used to detect the result.
RESULTS
The article illustrated that a large number of cancers had a higher expression of compared to the control tissues. And the immunohistochemical score (IHC score) of in cancer tissue was considerably elevated compared to that in normal tissues was elevated in thyroid carcinoma (THCA) tissue, besides, GEO analyses, qRT-PCR of tissues, and immunohistochemical staining were conducted to test the results. Finally, the Kaplan-Meier survival analysis illustrated that the increased expression of was correlated with a dismal prognosis-higher SEZ6L2 is associated with shorter survival. And the univariate analysis illustrated that T stage, SEZ6L2 and Pathologic stage were related to the overall survival (OS), multivariate analysis stated that elevated expression of was an independent risk factor that affected progression-free interval (PFI) (P<0.05). Consequently, we found that the expression of was correlated with tumor-infiltrating immune cells by TIMER.
CONCLUSIONS
was upregulated in patients with THCA and that increased expression of was related with clinical progression and was regarded as an independent risk factor for PFI. In THCA patients, the expression of could be a significant prognostic factor, which is expected to be a prospective biomarker for THCA in the future.
背景
癫痫相关6同源物（小鼠）样2（SEZ6L2）是一种1型跨膜蛋白，主要在大脑中表达。在最近的报道中，已发现SEZ6L2在某些癌症中过表达，并可驱动肿瘤进展。然而，其在甲状腺癌中的功能和机制仍不清楚。
方法
在本文中，我们在癌症基因组图谱（TCGA）上搜索了SEZ6L2在泛癌中的表达，并使用TIMER2方法评估了这些数据。然后，在人类蛋白质图谱（HPA）数据中收集癌组织中SEZ6L2的免疫组织化学评分（IHC评分）。并且我们使用CIBERSORT评估SEZ6L2表达水平与甲状腺乳头状癌（PTC）组织中各种免疫细胞数量之间的关联。最后，使用基因表达综合数据库（GEO）分析、组织的实时定量聚合酶链反应（qRT-PCR）和免疫组织化学染色来检测结果。
结果
本文表明，与对照组织相比，大量癌症中SEZ6L2的表达更高。与正常组织相比，癌组织中SEZ6L2的免疫组织化学评分（IHC评分）显著升高，甲状腺癌（THCA）组织中SEZ6L2升高，此外，进行了GEO分析、组织的qRT-PCR和免疫组织化学染色以验证结果。最后，Kaplan-Meier生存分析表明，SEZ6L2表达增加与预后不良相关——SEZ6L2水平越高，生存期越短。单因素分析表明，T分期、SEZ6L2和病理分期与总生存期（OS）相关，多因素分析表明，SEZ6L2表达升高是影响无进展生存期（PFI）的独立危险因素（P<0.05）。因此，我们通过TIMER发现SEZ6L2的表达与肿瘤浸润免疫细胞相关。
结论
THCA患者中SEZ6L2上调，其表达增加与临床进展相关，被视为PFI的独立危险因素。在THCA患者中，SEZ6L2的表达可能是一个重要的预后因素，有望在未来成为THCA的一种前瞻性生物标志物。
Zotero 插件